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Literature summary for 2.7.11.15 extracted from
- High affinity binding of beta-adrenergic receptor kinase to microsomal membranes. Modulation of the activity of bound kinase by heterotrimeric G protein activation (1996), J. Biol. Chem., 271, 985-994.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AlF4- | enhances beta-ARK activity upon stimulation of heterotrimeric G proteins | Bos taurus |  |
| G protein betagamma-subunit | activates, binding domain is localized to the C-terminal region of beta-ARK | Bos taurus | |
| mastoparan/guanosine 5'-(3-O-thio)triphosphate | enhances beta-ARK activity upon stimulation of heterotrimeric G proteins | Bos taurus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system | Bos taurus |
| cloning of GST-beta-ARK fusion proteins and expression in Escherichia coli AG1 | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| heparin | - |
Bos taurus | |
| additional information | marked inhibition of rhodopsin or synthetic peptide phosphorylation in the presence of increasing amounts of microsomal membranes, bound enzyme is less able to interact with its substrate | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | associates with intracellular microsomal membranes, mechanism, modulation of binding of enzyme, main determinants of binding appear to be localized to an 60 amino acid residue stretch, residues 88 to 145, G protein betagamma subunits are not the main anchor in the membranes | Bos taurus | - |
- |
| additional information | complex modulation of the subcellular distribution of beta-ARK | Bos taurus | - |
- |
| plasma membrane | soluble enzyme, that transiently translocates to the plasma membrane | Bos taurus | 5886 | - |
| soluble | soluble enzyme, that transiently translocates to the plasma membrane | Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | increases binding of beta-ARK to microsomal membranes | Bos taurus | |
| Mn2+ | Mn2+ can partially substitute Mg2+ in increasing the binding of beta-ARK to microsomal membranes | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | Bos taurus | desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| additional information | Bos taurus | beta-ARK activity is regulated by endogenous G proteins in different intracellular locations | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
beta-ARK 1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant beta-ARK 1 expressed in Sf9 cells | Bos taurus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + rhodopsin | light-dependent, actual substrate: light-bleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + RRREEEEESAAA | - |
Bos taurus | ? | - |
? | |
| additional information | beta-ARK interacts rapidly with a high affinity binding site present in salt-stripped rat liver microsomal membranes, modulation of binding of enzyme | Bos taurus | ? | - |
? | |
| additional information | phosphorylates G protein coupled receptors in an agonist-dependent manner | Bos taurus | ? | - |
? | |
| additional information | beta-ARK activity is regulated by endogenous G proteins in different intracellular locations | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
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