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Literature summary for 2.7.11.14 extracted from

  • Singh, P.; Wang, B.; Maeda, T.; Palczewski, K.; Tesmer, J.J.
    Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation (2008), J. Biol. Chem., 283, 14053-14062.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.14

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged wild-type and mutant enzymes in High Five insect cells using the baculovirus transfection system Bos taurus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, X-ray diffraction structure determination and analysis, molecular replacement Bos taurus

Protein Variants

Protein Variants Comment Organism
D164A site-directed mutagenesis, a dimer interface mutant showing only slightly altered kinetics compared to the wild-type enzyme Bos taurus
D164A/L166K site-directed mutagenesis, a dimer interface mutant showing reduced activity compared to the wild-type enzyme Bos taurus
D164A/W531A site-directed mutagenesis, inactive mutant Bos taurus
L166K site-directed mutagenesis, a dimer interface mutant showing reduced activity compared to the wild-type enzyme Bos taurus
L166K/W531A site-directed mutagenesis, inactive mutant Bos taurus
S5A site-directed mutagenesis, an N-terminal mutant showing reduced activity compared to the wild-type enzyme Bos taurus
S5D site-directed mutagenesis, inactive mutant Bos taurus
T8A site-directed mutagenesis, an N-terminal mutant showing reduced activity compared to the wild-type enzyme Bos taurus
T8D site-directed mutagenesis, inactive mutant Bos taurus
T8E site-directed mutagenesis, an N-terminal mutant showing reduced activity compared to the wild-type enzyme Bos taurus
W531A site-directed mutagenesis, inactive mutant Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Bos taurus
0.0015
-
 rhodopsin pH 7.5, 30°C, mutant T8A Bos taurus
0.0021
-
 rhodopsin pH 7.5, 30°C, wild-type enzyme Bos taurus
0.0031
-
 rhodopsin pH 7.5, 30°C, mutant D164A/L166K Bos taurus
0.0038
-
 rhodopsin pH 7.5, 30°C, mutant D164A Bos taurus
0.0048
-
 rhodopsin pH 7.5, 30°C, mutant T8E Bos taurus
0.0051
-
 rhodopsin pH 7.5, 30°C, mutant S5A Bos taurus
0.0067
-
 rhodopsin pH 7.5, 30°C, mutant L166K Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus P28327
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from High Five insect cells by nickel affinity chromatography and ion exchange chromatography Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
additional information nucleotide binding and conformational change, overview Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
More domain structure analysis, mass spectrometric analysis, overview Bos taurus

Synonyms

Synonyms Comment Organism
GRK1
-
 Bos taurus
More GRK1 is the founding member of the G protein-coupled receptor kinase, GRK kinase, family Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Bos taurus