Activating Compound | Comment | Organism | Structure |
---|---|---|---|
polycation | activates approximately 2fold | Bos taurus |
Application | Comment | Organism |
---|---|---|
medicine | mutation in RK is associated with the Oguchi disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
RK gene is located on chromosome 13q34 | Homo sapiens |
RK is cloned and expressed in SF9 cells | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chelator | - |
Bos taurus | |
Mg2+ | at high concentrations; requirement at lower concentrations | Bos taurus | |
polyanion | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | requirement | Bos taurus | |
Mg2+ | 1 mM required | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Rattus norvegicus | - |
ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | Homo sapiens | important enzyme of phototransduction | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | Bos taurus | involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction | ADP + phosphorhodopsin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation of RK causes a lower affinity of enzyme for photolyzed rhodopsin allowing dissociation | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
eye | retina | Homo sapiens | - |
eye | retina | Bos taurus | - |
pineal gland | - |
Rattus norvegicus | - |
pineal gland | - |
Bos taurus | - |
retina | - |
Homo sapiens | - |
retina | rod cell outer segment | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | 0.7 | - |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + peptide | acid-rich peptides, RK prefers acid residues localized to the C-terminal side of the serine | Bos taurus | ADP + phosphopeptide | - |
? | |
ATP + peptide | containing sites phosphorylated in rhodopsin | Bos taurus | ADP + phosphopeptide | - |
? | |
ATP + rhodopsin | - |
Rattus norvegicus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | preferred substrate: ATP | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | phosphorylates bovine rhodopsin | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | highly specific for photobleached rhodopsin | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | RK binds to the cytoplasmic loops of photolyzed rhodopsin, forming a stable complex, and then phosphorylating it at the C-terminus, phosphorylation at different sites, including Ser-334, Ser-338 and Ser342, may play different roles in phototransduction | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | domain structure | Bos taurus | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | important enzyme of phototransduction | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | involved in a mechanism for quenching or terminating the visual signal involving the interaction of metarhodopsin II with RK and arrestin, phosphorylation of light-activated rhodopsin by RK is the key step in the signal-termination reaction | Bos taurus | ADP + phosphorhodopsin | - |
? | |
GTP + rhodopsin | very poor substrate | Bos taurus | GDP + phosphorhodopsin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Bos taurus |
7.5 | - |
- |
Homo sapiens |
7.5 | - |
assay at, photolyzed rhodopsin substrate | Bos taurus |
8.5 | - |
assay at, synthetic peptide substrates | Bos taurus |