Application | Comment | Organism |
---|---|---|
medicine | mutations occuring in the variable region of RK C-terminus and catalytic domain cause Oguchi disease, an inherited form of stationary night blindness | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cDNAs encoding full-length RK and C-terminus-truncated mutant RK lacking the last 59 amino acids are cloned and expressed in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | 50 kDa C-terminus-truncated mutant RK lacking the last 59 amino acids shows abolished light-dependent translocation and is unable to phosphorylate photoactivated rhodopsin, but phosphorylates the small peptide substrate RRREEEEESAAA like wild-type RK | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | 0.0044 | rhodopsin | pH 7.5, 30°C | Homo sapiens | |
2 | - |
RRREEEEESAAA | pH 7.5, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | recombinant RK, in both plasma membranes and cytosolic fractions | Homo sapiens | 5829 | - |
plasma membrane | recombinant RK, in both plasma membranes and cytosolic fractions | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, Western blot analysis | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Homo sapiens | natural substrate is photoactivated rhodopsin | ADP + phosphorhodopsin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | C-terminus is required for phosphorylation of photo-activated rhodopsin and may be involved in interaction with it | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | specific for photoactivated rhodopsin, time-dependent phosphorylation, light-dependent translocation, i.e. association of the kinase with photoactivated rhodopsin | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + rhodopsin | natural substrate is photoactivated rhodopsin | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
ATP + RRREEEEESAAA | synthetic peptide substrate | Homo sapiens | ADP + RRREEEEE-(P)SAAA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, Western blot analysis | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |