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Literature summary for 2.7.11.14 extracted from
- Purification and characterization of rhodopsin kinase (1988), J. Biol. Chem., 263, 14067-14073.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-myo-Inositol 1-phosphate | about 20% activation, between 0.0003 mM and 0.1 mM, slightly inhibitory at higher concentrations | Bos taurus |  |
General Stability
| General Stability | Organism |
|---|---|
| dilution inactivates | Bos taurus |
| freezing at liquid N2 temperatures or -20°C, in water or inositol leads to 80% loss of activity, in sucrose to 20% loss of activity | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2',3'-dideoxyadenosine | - |
Bos taurus | |
| 3'-deoxyadenosine | - |
Bos taurus | |
| 5'-AMP | competitive inhibition with respect to ATP | Bos taurus | |
| 5'-deoxyadenosine | - |
Bos taurus | |
| adenosine | competitive inhibition with respect to ATP | Bos taurus | |
| adenosine 5'-monosulfate | - |
Bos taurus | |
| ADP | competitive inhibition with respect to ATP | Bos taurus | |
| ATP-analogues | weak or no inhibition, overview | Bos taurus | |
| cAMP | weak | Bos taurus | |
| D-myo-Inositol 1-phosphate | weak, above 0.1 mM, stimulates below | Bos taurus | |
| inositol triphosphate | weak | Bos taurus | |
| Mg2+ | at high concentrations; requirement at lower concentrations | Bos taurus | |
| additional information | little inhibition by S-adenosyl-L-methionine, coenzyme A, methylene adenosine 5'-triphosphate, imidoadenosine 5'-triphosphate, adenosine 9-arabinofuranoside 5'-monophosphate, GMP, adenosine N1-oxide; not inhibited by 2'-deoxyadenosine, adenosine 2',3'-monophosphate, adenosine 2'-deoxy-3,5-monophosphate, inosine monophosphate, guanosine, GDP, xanthosine 5'-monophosphate, hypoxanthine 9-arabinofuranoside, 1-N6-ethenoadenosine monophosphate, NAD+, NADH, NADP+, NADPH; not inhibited by cAMP; not inhibited by cGMP | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | 0.002 | ATP | pH 7.5, 25°C, at 1 mM Mg2+ | Bos taurus | |
| 0.004 | 0.0044 | rhodopsin | pH 7.5, 25°C, freshly bleached | Bos taurus | |
| 0.0052 | - |
ATP | pH 7.5, 25°C, at 10 mM Mg2+ | Bos taurus | |
| 1 | - |
GTP | pH 7.5, 25°C, at 2 mM Mg2+ | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | behaves as a soluble protein | Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | inhibition at high concentrations | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
gel filtration | Bos taurus |
| 70000 | - |
1 * 70000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | marked sensitivity to organic solvents, e.g. 5% ethanol reduces the activity by 45% within 2-3 min | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 1055fold, to near homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Bos taurus | - |
| retina | rod cell outer segment | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.96 | - |
pH 7.5, 25°C | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% adonitol, several months, stable | Bos taurus |
| -20°C, kinase solution, solid sucrose, initially loses 30% of activity, stable to further storage | Bos taurus |
| 4°C, crude extract, t1/2: 30 days, highly purified enzyme, t1/2: 3-5 h | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | specificity of ATP-binding site | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | preferred substrate: ATP | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| GTP + rhodopsin | very poor substrate | Bos taurus | GDP + phosphorhodopsin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
adenosine | pH 7.5, 25°C | Bos taurus | |
| 0.005 | - |
5'-AMP | pH 7.5, 25°C | Bos taurus | |
| 0.01 | - |
5'-deoxyadenosine | pH 7.5, 25°C | Bos taurus | |
| 0.012 | - |
ADP | pH 7.5, 25°C | Bos taurus | |
| 0.016 | - |
3'-deoxyadenosine | pH 7.5, 25°C | Bos taurus | |
| 0.065 | - |
2',3'-dideoxyadenosine | pH 7.5, 25°C | Bos taurus | |
| 0.08 | - |
adenosine 5'-monosulfate | pH 7.5, 25°C | Bos taurus | |
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