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Literature summary for 2.7.11.14 extracted from
- Rhodopsin kinase (1983), Methods Enzymol., 99, 362-366.
General Stability
| General Stability | Organism |
|---|---|
| monovalent cations, e.g. K+ or NH4+, and 15% glycerol stabilize to some extent | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | 1 mM, 50% inhibition | Bos taurus |  |
| adenosine | 1 mM, 50% inhibition | Bos taurus | |
| Digitonin | 0.1%, about 60% inhibition | Bos taurus | |
| emulphogene | BC720, 0.1%, about 60% inhibition | Bos taurus | |
| additional information | not inhibited by cAMP; not inhibited by cGMP; not inhibited by K+; not inhibited by spermidine | Bos taurus | |
| Na+ | 0.1 M, 90% inhibition | Bos taurus | |
| Zn2+ | 1 mM, 90% inhibition | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
ATP | pH 7.4, 37°C | Bos taurus | |
| 0.4 | - |
GTP | pH 7.4, 37°C | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Bos taurus | 16020 | - |
| membrane | located on the external surface of the disc membrane, the affinity of membrane for the enzyme increases upon photobleaching of rhodopsin | Bos taurus | 16020 | - |
| membrane | rod membranes | Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | 5 M, almost complete denaturation of enzyme | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | enzyme itself is phosphorylated by ATP | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| eye | retina | Bos taurus | - |
| eye | dark-adapted | Bos taurus | - |
| retina | rod cell outer segment | Bos taurus | - |
| retina | from dark-adapted eyes | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.026 | 0.033 | pH 7.4, 37°C | Bos taurus |
| 0.028 | - |
pH 7.4, 37°C | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C, 0.1 M KCl, 1 week, 50% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | highly specific for rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
ir | |
| ATP + rhodopsin | light-dependent phosphorylation | Bos taurus | ADP + phosphorhodopsin | - |
ir | |
| ATP + rhodopsin | phosphorylates bovine rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
ir | |
| ATP + rhodopsin | incorporation of 5-7 phosphate groups/mol rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
ir | |
| ATP + rhodopsin | catalyzes the transfer of the terminal gamma phosphate group of ATP to the opsin protein | Bos taurus | ADP + phosphorhodopsin | - |
ir | |
| ATP + rhodopsin | phosphorylates serine and threonine residues in the carboxy-terminal region of opsin peptide | Bos taurus | ADP + phosphorhodopsin | - |
ir | |
| GTP + rhodopsin | can replace ATP to a lesser extent | Bos taurus | GDP + phosphorhodopsin | - |
? | |
| additional information | not: protamine | Bos taurus | ? | - |
? | |
| additional information | not: casein, phosvitin, histones | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
2 optima: pH 6 and pH 7.5 | Bos taurus |
| 7.4 | - |
assay at | Bos taurus |
| 7.5 | - |
2 optima, pH 6 and pH 7.5 | Bos taurus |
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Release 2023.1 (January 2023)
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