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Literature summary for 2.7.11.12 extracted from

  • Byun, J.A.; Van, K.; Huang, J.; Henning, P.; Franz, E.; Akimoto, M.; Herberg, F.W.; Kim, C.; Melacini, G.
    Mechanism of allosteric inhibition in the Plasmodium falciparum cGMP-dependent protein kinase (2020), J. Biol. Chem., 295, 8480-8491 .
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
beta-phenyl-1,N2-ethenoguanosine-3',5'-cyclic monophosphate
-
 Plasmodium falciparum
cGMP
-
 Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
cyclic nucleotide-binding domain D construct (residues 401-542) is expressed in Escherichia coli BL21(DE3) cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
8-(2-[7-nitro-4-benzofurazanyl]aminoethylthio)guanosine-3',5'-cyclic monophosphate the compound significantly reduces the kinase activity of the enzyme and is the most potent inhibitor Plasmodium falciparum
8-(4-chlorophenylthio)guanosine-3',5'-cyclic monophosphorothioate the compound significantly reduces the kinase activity of the enzyme Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2-Sepharose resin column chromatography and Superdex 75 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + PKStide PKStide is GRTGRRNSI Plasmodium falciparum ADP + phosphorylated PKStide
-
 ?

Synonyms

Synonyms Comment Organism
PKG
-
 Plasmodium falciparum