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Literature summary for 2.7.11.12 extracted from

  • Qin, L.; Reger, A.S.; Guo, E.; Yang, M.P.; Zwart, P.; Casteel, D.E.; Kim, C.
    Structures of cGMP-dependent protein kinase (PKG) Ialpha leucine zippers reveal an interchain disulfide bond important for dimer stability (2015), Biochemistry, 54, 4419-4422.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 40% (w/v) PEG 200, 200 mM lithium sulfate, and 0.1 mM tris base/hydrochloric acid (pH 8.5) at 22°C Homo sapiens

Protein Variants

Protein Variants Comment Organism
C42L the mutant shows similar temperature factors, which are consistently lower than those of wild type dimers Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Homo sapiens
-
 ADP + phosphorylated protein
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13976
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
 Homo sapiens ADP + phosphorylated protein
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
cGMP-dependent protein kinase Ialpha
-
 Homo sapiens
cyclic GMP-dependent protein kinase
-
 Homo sapiens
PKG Ialpha
-
 Homo sapiens