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Literature summary for 2.7.11.12 extracted from

  • Fryer, B.H.; Wang, C.; Vedantam, S.; Zhou, G.L.; Jin, S.; Fletcher, L.; Simon, M.C.; Field, J.
    cGMP-dependent protein kinase phosphorylates p21-activated kinase (Pak) 1, inhibiting Pak/Nck binding and stimulating Pak/vasodilator-stimulated phosphoprotein association (2006), J. Biol. Chem., 281, 11487-11495.
    View publication on PubMed
Search for more literature for 2.7.11.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of isozyme PKG1beta in COS-7 or 293T cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
8-(4-chlorophenylthio)guanosine 3',5'-cyclic monophosphate
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + p21-activated kinase Homo sapiens PKG phosphorylation of p21-activated kinase, i.e. Pak, regulates HeLa cell and human umbilical vein endothelial cell morphology and cellular remodeling, time course of Pak1 activation in vivo, overview, PKG stimulates association of vasodilator-stimulated phosphoprotein VASP with Pak ADP + phosphorylated p21-activated kinase
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozyme PKG 1beta
-

Source Tissue

Source Tissue Comment Organism Textmining
endothelial cell HUVEC Homo sapiens
-
HeLa cell
-
 Homo sapiens
-
umbilical vein HUVEC Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + p21-activated kinase PKG phosphorylation of p21-activated kinase, i.e. Pak, regulates HeLa cell and human umbilical vein endothelial cell morphology and cellular remodeling, time course of Pak1 activation in vivo, overview, PKG stimulates association of vasodilator-stimulated phosphoprotein VASP with Pak Homo sapiens ADP + phosphorylated p21-activated kinase
-
 ?
ATP + p21-activated kinase activation of the wild-type p21-activated kinase, i.e. Pak1, PKG phosphorylates wild-type and mutant K299R Pak, PKG phosphorylates especially N-terminal amino acids 1–74, e.g. Ser21, and does not phosphorylate a fragment encompassing amino acids 67–149 and only weakly phosphorylated fragments from the central region of Pak1 encompassing amino acids 147–231 and the C-terminal catalytic region, amino acids 231–544, no activity with Pak1 mutant S21A, overview Homo sapiens ADP + phosphorylated p21-activated kinase
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the AGC kinase family Homo sapiens
PKG
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens