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Literature summary for 2.7.11.12 extracted from

  • MacPherson, M.R.; Lohmann, S.M.; Davies, S.A.
    Analysis of Drosophila cGMP-dependent protein kinases and assessment of their in vivo roles by targeted expression in a renal transporting epithelium (2004), J. Biol. Chem., 279, 40026-40034.
    View publication on PubMed
Search for more literature for 2.7.11.12

Activating Compound

Activating Compound Comment Organism Structure
cGMP stimulates DG2P1 and DG2P2, and DG1, the latter maximally at 20 mM Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment) Organism
in vitro transcription of DG2P1 and DG2P2, and DG1 by RT-PCR of cDNAs from tubule cells, targeted expression of DG2P1 and DG2P2, and DG1 in malpigian tubule using the UAS/GAL4 system, differential localization of all 3 enzyme forms in transgenic flies Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic flies by targeted expression of DG2P1 and DG2P2, and DG1 in malpigian tubule using the UAS/GAL4 system, differential localization of all 3 enzyme forms in transgenic flies, phenotypes, overview Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
Rp-8-pCPT-cGMP i.e. 8-(4-chlorophenylthio)guanosine-3',5'-cyclic monophosphorothionate Rp isomer, inhibits DG2P1 and DG2P2 at 0.01 and 0.1 mM, respectively Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol DG1 Drosophila melanogaster 5829
-
plasma membrane DG2P1 and DG2P2 Drosophila melanogaster 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required by DG2P1 and DG2P2, and DG1 Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, recombinant DG1, SDS-PAGE, x * 84000, recombinant DG2P1 and DG2P2, SDS-PAGE Drosophila melanogaster
84000
-
 x * 65000, recombinant DG1, SDS-PAGE, x * 84000, recombinant DG2P1 and DG2P2, SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster DG2P1 and DG2P2 are involved in behaviour and epithelial transport ?
-
 ?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
 gene dg2 or for encoding two enzyme forms DG2P1 and DG2P2, and gene dg1 encoding enzyme form DG1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation by DG1 Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
epithelium
-
 Drosophila melanogaster
-
head of the fly Drosophila melanogaster
-
malpighian tubule
-
 Drosophila melanogaster
-
SCHNEIDER-2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone DG1 Drosophila melanogaster ADP + phosphorylated histone
-
 ?
ATP + RKRSRAE cGK-specific peptide substrate Drosophila melanogaster ADP + RKRS(P)RAE
-
 ?
additional information DG2P1 and DG2P2 are involved in behaviour and epithelial transport Drosophila melanogaster ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, recombinant DG1, SDS-PAGE, x * 84000, recombinant DG2P1 and DG2P2, SDS-PAGE Drosophila melanogaster

Synonyms

Synonyms Comment Organism
CGK
-
 Drosophila melanogaster
DG2P1
-
 Drosophila melanogaster
DG2P2
-
 Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Drosophila melanogaster