Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cGMP | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
placental PKG Ibeta, expression of wild-type and mutant enzymes in Escherichia coli and in Spodoptera frugiperda Sf9 cells using the baculovirus infection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I24A | site-directed mutagenesis, unaltered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L17A | site-directed mutagenesis, unaltered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L17A/I24A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L17A/I24A/L31A/I38A/L45A/I52A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L31A/I38A | site-directed mutagenesis, unaltered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L31A/I38A/L45A/I52A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L3A/L10A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L3A/L10A/L17A/I24A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
L3A/L10A/L45A/I52A | site-directed mutagenesis, altered dimerization and molecular weight compared to the wild-type enzyme | Homo sapiens |
additional information | construction of a PKG Ibeta leucine zipper mutant, and of the deletion mutant DELTA1-52 lacking the N-terminal part, mutants show slightly altered properties, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and dissociation constants of cGMP | Homo sapiens | |
0.0104 | - |
ATP | pH 7.4, 30°C, recombinant PKG Ibeta deletion mutant DELTA1-52 | Homo sapiens | |
0.0133 | - |
ATP | pH 7.4, 30°C, recombinant wild-type PKG Ibeta | Homo sapiens | |
0.0139 | - |
ATP | pH 7.4, 30°C, recombinant PKG Ibeta leucine zipper mutant | Homo sapiens | |
0.0263 | - |
RKRSRAE | pH 7.4, 30°C, recombinant PKG Ibeta deletion mutant DELTA1-52 | Homo sapiens | |
0.0304 | - |
RKRSRAE | pH 7.4, 30°C, recombinant wild-type PKG Ibeta | Homo sapiens | |
0.0313 | - |
RKRSRAE | pH 7.4, 30°C, recombinant PKG Ibeta leucine zipper mutant | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
recombinant deletion mutant DELTA1-52, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
86000 | - |
recombinant PKG Ibeta mutant L17A/I24A, monomeric form at 0.95 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
88000 | - |
recombinant PKG Ibeta mutant L31A/I38A, monomeric form at 0.95 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
89000 | - |
recombinant PKG Ibeta mutant L3A/L10A/L45A/I52A, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
92000 | - |
recombinant PKG Ibeta mutant L31A/I38A/L45A/I52A, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
96000 | - |
recombinant PKG Ibeta mutant L3A/L10A/L17A/I24A, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
98000 | - |
recombinant PKG Ibeta leucine zipper mutant, monomeric form, sedimentation equilibrium analysis | Homo sapiens |
120000 | - |
recombinant PKG Ibeta mutant L17A/I24A, dimeric form at 51 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
140000 | - |
recombinant PKG Ibeta mutant L31A/I38A, dimeric form at 51 nM concentration, sedimentation equilibrium analysis | Homo sapiens |
170000 | - |
recombinant PKG Ibeta mutants L3A/L10A, L17A, and I24A, dimeric forms, sedimentation equilibrium analysis | Homo sapiens |
180000 | - |
recombinant wild-type PKG Ibeta, dimeric form, sedimentation equilibrium analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
females | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant PKG Ibeta from Sf9 insect cells by 8-amino-hexylamino-cAMP affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RKRSRAE | commercial synthetic peptide substrate | Homo sapiens | ADP + RKRS(P)RAE | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | dimerization of PKG Ibeta via the N-terminal dimerization domain containing an extensive leucine zipper motif modifying enzyme activity | Homo sapiens |
More | analysis of the leucine zipper motif within the N-terminal dimerization domain, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cGKIbeta | - |
Homo sapiens |
cGMP-dependent protein kinase Ibeta | - |
Homo sapiens |
PKG Ibeta | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |