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Literature summary for 2.7.11.11 extracted from

  • Wu, Z.; Jin, Y.; Chen, B.; Gugger, M.K.; Wilkinson-Johnson, C.L.; Tiambeng, T.N.; Jin, S.; Ge, Y.
    Comprehensive characterization of the recombinant catalytic subunit of cAMP-dependent protein kinase by top-down mass spectrometry (2019), J. Am. Soc. Mass Spectrom., 30, 2561-2570 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant PKA C-subunit is expressed in Escherichia coli Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P17612
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Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation sites of the most abundant PKA C-subunit proteoform are characterized simultaneously using tandem MS methods. Four sites are unambiguously identified as Ser10, Ser11, Ser18, and Ser30 and the remaining phosphorylation sites are localized to Ser2/Ser3, Ser358/Thr368, and Thr[215-224]Tyr in the PKA C-subunit sequence with a 20mer 6xHis-tag added at the N-terminus. Four of these seven phosphorylation sites are located at the 6xHis-tag Mus musculus

Purification (Commentary)

Purification (Comment) Organism
the recombinant PKA C-subunit is purified via His-tag affinity purification Mus musculus

Synonyms

Synonyms Comment Organism
PKA Calpha
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Mus musculus