Cloned (Comment) | Organism |
---|---|
the recombinant PKA C-subunit is expressed in Escherichia coli | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P17612 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation sites of the most abundant PKA C-subunit proteoform are characterized simultaneously using tandem MS methods. Four sites are unambiguously identified as Ser10, Ser11, Ser18, and Ser30 and the remaining phosphorylation sites are localized to Ser2/Ser3, Ser358/Thr368, and Thr[215-224]Tyr in the PKA C-subunit sequence with a 20mer 6xHis-tag added at the N-terminus. Four of these seven phosphorylation sites are located at the 6xHis-tag | Mus musculus |
Purification (Comment) | Organism |
---|---|
the recombinant PKA C-subunit is purified via His-tag affinity purification | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
PKA Calpha | - |
Mus musculus |