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Literature summary for 2.7.11.11 extracted from
- Ras/cAMP-dependent protein kinase (PKA) regulates multiple aspects of cellular events by phosphorylating the Whi3 cell cycle regulator in budding yeast (2013), J. Biol. Chem., 288, 10558-10566.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| co-overexpression of GST-tagged enzyme with GST-tagged Whi3 protein in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [Whi3 cell cycle regulator protein] | Saccharomyces cerevisiae | phosphorylation of Ser568 leads to inhibition of Whi3 | ADP + [Whi3 cell cycle regulator phosphoprotein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme and GST-tagged Whi3 protein from Saccharomyces cerevisiae by glutathione affinity chromatography | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [Whi3 cell cycle regulator protein] | phosphorylation of Ser568 leads to inhibition of Whi3 | Saccharomyces cerevisiae | ADP + [Whi3 cell cycle regulator phosphoprotein] | - |
? | |
| ATP + [Whi3 cell cycle regulator protein] | phosphorylation of Ser568 | Saccharomyces cerevisiae | ADP + [Whi3 cell cycle regulator phosphoprotein] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PKA | - |
Saccharomyces cerevisiae |
| Ras/cAMP-dependent protein kinase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the phosphomimetic S568D mutation of Whi3 prevents the developmental fate switch to sporulation or invasive growth | Saccharomyces cerevisiae |
| physiological function | phosphorylation of Whi3 by PKA leads to its decreased interaction with CLN3 G1 cyclin mRNA and is required for the promotion of G1/S progression, phosphorylation state of Ser568 in Whi3 affects G1/S transition by modulating CLN2 transcription. PKA causes a decrease in cell size by downregulating Whi3 function. Implication of PKA-mediated modulation of Whi3 in multiple cellular events | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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