Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | activation of protein kinase A involves the synergistic binding of cAMP to two cAMP binding sites on the inhibitory R subunit, causing release of the C subunit, which subsequently can carry out catalysis | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a construct RIalpha-(98-381) subunit comprising both cyclic nucleotide binding domains, in the presence and absence of cAMP, mapping the effects of cAMP binding at single residue resolution, NMR study, overview | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Leu-Arg-Arg-Ala-Ser-Leu-Gly | commercial artificial heptapeptide substrate kemptide | Bos taurus | ADP + Leu-Arg-Arg-Ala-phospho-Ser-Leu-Gly | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Bos taurus |
protein kinase A | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |
General Information | Comment | Organism |
---|---|---|
additional information | several conformationally disordered regions in free RIalpha become structured upon cAMP binding, including the interdomain alphaC:A and alphaC':A helices that connect cyclic nucleotide binding domains A and B and are primary recognition sites for the C subunit, unidirectional allosteric communication between the sites, Trp262, which lines the cyclic nucleotide binding A site but resides in the sequence of domain B, is an important structural determinant for intersite communication, overview | Bos taurus |