Any feedback?
Literature summary for 2.7.11.11 extracted from
- Characterization of the regulatory subunit of Yarrowia lipolytica cAMP-dependent protein kinase. Evidences of a monomeric protein (2011), Arch. Biochem. Biophys., 509, 66-75.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene RKA1, regulatory subunit, located in the F chromosome, expression of His6-tagged R subunit in Escherichia coli strain BL21(DE3) | Yarrowia lipolytica |
| gene TPK1 | Yarrowia lipolytica |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Yarrowia lipolytica |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42800 | - |
1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation | Yarrowia lipolytica |
| 48000 | - |
1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE | Yarrowia lipolytica |
| 90000 | - |
holoenzyme, gel filtration | Yarrowia lipolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | C3W7W0 | catalytic subunit, gene TPK1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits | - |
| Yarrowia lipolytica | F2XG10 | regulatory subunit, gene RKA1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the catalytic subunits phosphorylate the regulatory subunits | Yarrowia lipolytica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native catalytic subunit from by anion exchange and cAMP affinity chromatography | Yarrowia lipolytica |
| recombinant His6-tagged R subunit from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography | Yarrowia lipolytica |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation | Yarrowia lipolytica |
| monomer | 1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE | Yarrowia lipolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PKA | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Yarrowia lipolytica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Yarrowia lipolytica | regulatory subunit, sequence calculation | - |
5.18 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
