Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dependent on | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expression of PKA catalytic subunit Calpha as His-tagged protein in Escherichia coli strain BL21(DE3) and of HA-tagged wild-type and mutant PKA Calpha in COS cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K72A | site-directed mutagenesis, inactive unphosphorylated mutant | Mus musculus |
K72H | site-directed mutagenesis, inactive unphosphorylated mutant | Mus musculus |
K72M | site-directed mutagenesis, inactive unphosphorylated mutant | Mus musculus |
K72R | site-directed mutagenesis, inactive unphosphorylated mutant | Mus musculus |
S338A | site-directed mutagenesis, mutant is phosphorylated at T197, reduced activity compared to the wild-type enzyme | Mus musculus |
T197A | site-directed mutagenesis, mutant is not phosphorylated, inactive mutant | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H-89 | inhibits autophosphorylation of the catalytic subunit C, reversible | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation at T197 by exogenous kinase PDK-I, the enzyme performs autophosphorylation at K72, S338 and T197, the latter is phosphorylated first and required for activity, phosphorylation of the other 2 positions take place only if T197 is already phosphorylated, mechanism | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Kemptide | LRRASLG peptides substrate | Mus musculus | ADP + phosphorylated Kemptide | LRRA-phosphoserine-LG | ? | |
additional information | the enzyme performs autophosphorylation at K72, S338, and T197 | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |