Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dependent on | Cricetulus griseus |
Application | Comment | Organism |
---|---|---|
drug development | recombinant PKA can be mutated to resemble Akt/PKB, which cannot be easily recombinantly expressed, mutant PKA can be used as surrogate enzyme for Akt/PKB in drug design | Cricetulus griseus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant PKAs in Escherichia coli strain BL21(DE3) | Cricetulus griseus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | removal of residues S10, S139, and S338 plus Akt/PKB-like mutations of the ATP binding site render PKA to a Akt/PKB similar enzyme, overview | Cricetulus griseus |
S10A/S139D/S338D | site-directed mutagenesis, mutant shows activity and properties similar to the wild-type enzyme | Cricetulus griseus |
S10A/S139D/T197D/S338D | site-directed mutagenesis, mutant shows reduced expression level in Escherichia coli and impaired folding | Cricetulus griseus |
S10A/S139D/T197E/S338D | site-directed mutagenesis, mutant shows reduced expression level in Escherichia coli and impaired folding | Cricetulus griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation at Ser10, Ser139, Thr197, and Ser338 | Cricetulus griseus |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant PKAs from Escherichia coli strain BL21(DE3) | Cricetulus griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | - |
Cricetulus griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Kemptide | activity of catalytic PKA subunit | Cricetulus griseus | ADP + phosphorylated Kemptide | - |
? | |
additional information | the enzyme performs autophosphorylation at Ser10, Ser139, Thr197, and Ser338 | Cricetulus griseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure of catalytic subunit C with ATP binding cleft and activation loop, overview | Cricetulus griseus |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Cricetulus griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Cricetulus griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Cricetulus griseus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Cricetulus griseus |