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Literature summary for 2.7.11.11 extracted from

  • Hollinger, S.; Ramineni, S.; Hepler, J.R.
    Phosphorylation of RGS14 by protein kinase A potentiates its activity toward Galphai (2003), Biochemistry, 42, 811-819.
    View publication on PubMed
Search for more literature for 2.7.11.11

Activating Compound

Activating Compound Comment Organism Structure
cAMP dependent on, stimulates phosphorylation of RGS protein Rattus norvegicus
additional information forskolin stimulates the adenylyl cyclase, which activates the enzyme by increased cAMP level Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
H89 specific inhibition Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + RGS protein Rattus norvegicus recombinant HA-tagged substrate expressed in B35 cells, phosphorylation of RGS14 by PKA potentiates its activity toward Galphai-GDP ADP + RGS protein phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate the substrate binds to the regulatory subunit which is released from the catalytic subunit for enzyme activity, mechanism and structure-function relationship Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
B35 cell neuroblastoma cell line Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RGS protein recombinant HA-tagged substrate expressed in B35 cells, phosphorylation of RGS14 by PKA potentiates its activity toward Galphai-GDP Rattus norvegicus ADP + RGS protein phosphate
-
 ?
ATP + RGS protein i.e. regulator of G protein signaling protein, recombinant His-tagged substrate R14-RSG residues 1-205 and 299-544, phosphorylation of wild-type R14-RSG at Ser258 and Thr494 Rattus norvegicus ADP + RGS protein phosphate
-
 ?

Subunits

Subunits Comment Organism
More the substrate binds to the regulatory subunit which is released from the catalytic subunit for enzyme activity Rattus norvegicus

Synonyms

Synonyms Comment Organism
PKA
-
 Rattus norvegicus
protein kinase A
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus