Literature summary for 2.7.11.11 extracted from

Diaz-Enrich, M.J.; Ibarguren, I.; Hellman, U.; Villamarin, J.A.
Characterization of a type I regulatory subunit of cAMP-dependent protein kinase from the bivalve mollusk Mytilus galloprovincialis (2003), Arch. Biochem. Biophys., 416, 119-127.

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Activating Compound

Activating Compound	Comment	Organism	Structure
cAMP	dependent on	Mytilus galloprovincialis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mytilus galloprovincialis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	1 * 54000, Rmyt2, reducing or nonreducing SDS-PAGE, 1 * 54000, Rmyt1, reducing SDS-PAGE	Mytilus galloprovincialis
54000	-	2 * 54000, (Rmyt1)2, nonreducing SDS-PAGE	Mytilus galloprovincialis

Organism

Organism	UniProt	Comment	Textmining
Mytilus galloprovincialis	-	bivalve mollusk, mussel	-

Purification (Commentary)

Purification (Comment)	Organism
purification of the type 1 regulatory subunit Rmyt1 from mussel by DEAE ion exchange and immunoaffinity chromatography, the latter based on antibodies and cGMP-agarose, and gel filtration	Mytilus galloprovincialis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + Kemptide	activity of the catalytic subunit C	Mytilus galloprovincialis	ADP + Kemptide phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 54000, (Rmyt1)2, nonreducing SDS-PAGE	Mytilus galloprovincialis
monomer	1 * 54000, Rmyt2, reducing or nonreducing SDS-PAGE, 1 * 54000, Rmyt1, reducing SDS-PAGE	Mytilus galloprovincialis
More	2 isoforms of regulatory subunit termed Rmyt1, type 1, and Rmyt2, one form of catalytic subunit C, peptide mapping of Rmyt1	Mytilus galloprovincialis

Synonyms

Synonyms	Comment	Organism
PKA	-	Mytilus galloprovincialis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Mytilus galloprovincialis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Mytilus galloprovincialis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mytilus galloprovincialis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Mytilus galloprovincialis	regulatory subunit Rmyt2, isoelectric focusing	-	4.7
Mytilus galloprovincialis	regulatory subunit Rmyt1, isoelectric focusing	-	5

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Release 2023.1 (January 2023)