Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-bromo-cAMP | activates to same extent as cAMP | Komagataella pastoris | |
8-chloro-cAMP | activates to same extent as cAMP | Komagataella pastoris | |
cAMP | dependent on, degree of enzyme activation by cAMP or cGMP depends on the substrate | Komagataella pastoris | |
cGMP | activates, degree of enzyme activation by cAMP or cGMP depends on the substrate | Komagataella pastoris | |
additional information | no stimulation by N6,2'-O-dibutyryl-cAMP | Komagataella pastoris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Komagataella pastoris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, about, regulatory subunit RII, SDS-PAGE | Komagataella pastoris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Komagataella pastoris JC100 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme performs autophosphorylation | Komagataella pastoris |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate fractionation, dialysis, DEAE ion exchange and P11 cellulose chromatography, and hydroxyapatite chromatography, concentration steps on PEG20000 | Komagataella pastoris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | enzyme activity is preferably activated by cAMP, 25% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated casein | - |
? | |
ATP + casein | enzyme activity is preferably activated by cAMP, 25% lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated casein | - |
? | |
ATP + histone H2A | enzyme activity is preferably activated by cAMP, 40% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated histone H2A | - |
? | |
ATP + histone H2A | enzyme activity is preferably activated by cAMP, 40% lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated histone H2A | - |
? | |
ATP + histone H3S | enzyme activity is preferably activated by cAMP, 60% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated histone H3S | - |
? | |
ATP + histone H3S | enzyme activity is preferably activated by cAMP, 60% lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated histone H3S | - |
? | |
ATP + Kemptide | enzyme activity is preferably activated by cAMP, 80% lower activity with cGMP | Komagataella pastoris | ADP + Kemptide phosphate | - |
? | |
ATP + maltose binding protein | enzyme activity is preferably activated by cAMP, 35% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated maltose binding protein | - |
? | |
ATP + phosvitin | enzyme activity is preferably activated by cAMP, 33% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated phosvitin | - |
? | |
ATP + protamine sulfate | enzyme activity is about equally activated by cAMP and cGMP, and derivatives of cAMP | Komagataella pastoris | ADP + phosphoprotamine sulfate | - |
? | |
ATP + pyruvate kinase | enzyme activity is about equally activated by cAMP and cGMP | Komagataella pastoris | ADP + phosphorylated pyruvate kinase | - |
? | |
ATP + ribosomal protein S6 | substrate from purified Saccharomyces cerevisiae ribosomes, enzyme activity is preferably activated by cAMP, lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated ribosomal protein S6 | - |
? | |
ATP + ribosomal protein S6 | substrate from purified Saccharomyces cerevisiae ribosomes, enzyme activity is preferably activated by cAMP, lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated ribosomal protein S6 | - |
? | |
additional information | the enzyme performs autophosphorylation, substrate specificity depends on activating agents cAMP or cGMP, overview | Komagataella pastoris | ? | - |
? | |
additional information | the enzyme performs autophosphorylation, substrate specificity depends on activating agents cAMP or cGMP, overview | Komagataella pastoris JC100 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about, regulatory subunit RII, SDS-PAGE | Komagataella pastoris |
Synonyms | Comment | Organism |
---|---|---|
cyclic AMP dependent protein kinase | - |
Komagataella pastoris |
PKA | - |
Komagataella pastoris |
protein kinase A | - |
Komagataella pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Komagataella pastoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Komagataella pastoris |