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Literature summary for 2.7.11.10 extracted from

  • Tegethoff, S.; Behlke, J.; Scheidereit, C.
    Tetrameric oligomerization of IkappaB kinase gamma (IKKgamma) is obligatory for IKK complex activity and NF-kappaB activation (2003), Mol. Cell. Biol., 23, 2029-2041.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of HA-tagged IKKbeta and GST-fusion IKKgamma in 293 cells, expression of GST-fusion or His-tagged IKKgamma protein in Escherichia coli strain BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of IKKgamma peptide fragements and sequence deletion mutants for determination of intermolecular interaction of IKKgamma required for 2-step tetramerization, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [IkappaB protein] Homo sapiens
-
ADP + [IkappaB phosphoprotein]
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion IKKgamma protein from Escherichia coli strain BL21 by glutathione affinity and anion exchange chromatography, and gel filtration, recombinant His-tagged IKKgamma protein from Escherichia coli strain BL21 by nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [IkappaB protein]
-
Homo sapiens ADP + [IkappaB phosphoprotein]
-
?

Subunits

Subunits Comment Organism
More 2-step tetrameric oligomerization of IKKgamma, mediated by C-terminal coiled-coil domains, is obligatory for IKK complex phosphorylation activity and NF-kappaB activation in vivo, the IKK complex consists of 3 subunits: catalytic IKKalpha and IKKbeta, and noncatalytic IKKgamma, which bind to each other independently of IKKgamma oligomerization status, overview Homo sapiens

Synonyms

Synonyms Comment Organism
IKK complex
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens