Cloned (Comment) | Organism |
---|---|
coexpression of HA-tagged IKKbeta and GST-fusion IKKgamma in 293 cells, expression of GST-fusion or His-tagged IKKgamma protein in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of IKKgamma peptide fragements and sequence deletion mutants for determination of intermolecular interaction of IKKgamma required for 2-step tetramerization, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [IkappaB protein] | Homo sapiens | - |
ADP + [IkappaB phosphoprotein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion IKKgamma protein from Escherichia coli strain BL21 by glutathione affinity and anion exchange chromatography, and gel filtration, recombinant His-tagged IKKgamma protein from Escherichia coli strain BL21 by nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [IkappaB protein] | - |
Homo sapiens | ADP + [IkappaB phosphoprotein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 2-step tetrameric oligomerization of IKKgamma, mediated by C-terminal coiled-coil domains, is obligatory for IKK complex phosphorylation activity and NF-kappaB activation in vivo, the IKK complex consists of 3 subunits: catalytic IKKalpha and IKKbeta, and noncatalytic IKKgamma, which bind to each other independently of IKKgamma oligomerization status, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IKK complex | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |