Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | IKKgamma phosphorylation by IKKbeta is induced by the cytokine TNF-R1, IKKbeta is recruited to the cytoplasmic tail of TNF-R1 by adaptor proteins, such as RIP | Homo sapiens | |
NEMO | i.e. NF-kappaB essential modifier, complexing with IkappaB kinase-gamma | Homo sapiens | |
Tax | viral oncoprotein, activates by direct binding, involved in ubiquitination and phosphorylation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
coexpression of FLAG-atgged wild-type and mutant IKKbeta proteins with T7-tagged IKKgamma and HA-tagged ubiquitin in 293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K106R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K147R | site-directed mutagenesis, reduced monoubiquitination of IKKbeta mutant | Homo sapiens |
K163R | site-directed mutagenesis, monoubiquitination-defective mutant of IKKbeta retaining kinase activity in Tax-expressing cells | Homo sapiens |
K171R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K18R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K198R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K234R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K238R | site-directed mutagenesis, monoubiquitination-defective mutant of IKKbeta | Homo sapiens |
K254R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K275R | site-directed mutagenesis, ubiquitination and phosphorylation of the mutant is unaltered compared to the wild-type IKKbeta | Homo sapiens |
K44R | site-directed mutagenesis, monoubiquitination-defective mutant of IKKbeta | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [IkappaBbeta protein] | Homo sapiens | - |
ADP + [IkappaBbeta phosphoprotein] | - |
? | |
additional information | Homo sapiens | IKKbeta regulates the translocation of NF-kappaB from cytoplasm to nucleus earmarking the transcription factor for polyubiquitination and proteasome-mediated degradation, the cytokine TNFalpha-induced T-loop-phosphorylated IKKbeta becomes monoubiquitinated at Lyk163 proximal to the T-loop, mechanism of post-translational crosstalk, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | IKKbeta is phosphorylated at the T loop residues Ser171 and Ser181, chronic phosphorylation leads to attachment of one ubiquitin molecule at nearby Lys163, which in turn modulates the phosphorylation status at select C-terminal serines | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [GST-IkappaBalpha1-54 protein] | - |
Homo sapiens | ADP + [GST-IkappaBalpha1-54 phosphoprotein] | - |
? | |
ATP + [IkappaBbeta protein] | - |
Homo sapiens | ADP + [IkappaBbeta phosphoprotein] | - |
? | |
additional information | IKKbeta regulates the translocation of NF-kappaB from cytoplasm to nucleus earmarking the transcription factor for polyubiquitination and proteasome-mediated degradation, the cytokine TNFalpha-induced T-loop-phosphorylated IKKbeta becomes monoubiquitinated at Lyk163 proximal to the T-loop, mechanism of post-translational crosstalk, overview | Homo sapiens | ? | - |
? | |
additional information | TNF-R1-activated IKKbeta phosphorylates IKKgamma and IKKalpha, autophosphorylation patterns involving K163 of IKKbeta, regulation, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | IKKbeta contains a leucine zipper, a T-loop, and a HLH domain important for phosphorylation and ubiquitination, the IKK complex consists of 3 subunits: catalytic IKKalpha and IKKbeta, and noncatalytic, regulatory NEMO/IKKgamma | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IKKbeta | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |