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Literature summary for 2.7.11.10 extracted from
- Tumor necrosis factor-alpha-induced IKK phosphorylation of NF-kappaB p65 on serine 536 is mediated through the TRAF2, TRAF5, and TAK1 signaling pathway (2003), J. Biol. Chem., 278, 36916-36923.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | TNFalpha, TGF-beta-activated kinase 1 TAK1, IKKalpha subunit and IKKbeta subunit induce IKK phosphorylation activity, overview | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant IKKbeta and IKKalpha in HeLa cells and in HEK293 cells, expression of His-tagged IKKbeta in Spodoptera frugiperda Sf21 cells via baculovirus infection | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K44M | site-directed mutagenesis, inactive IKKalpha mutant | Homo sapiens |
| K44M | site-directed mutagenesis, inactive IKKbeta mutant | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Bay 11-7082 | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [IkappaB protein] | Homo sapiens | - |
ADP + [IkappaB phosphoprotein] | - |
? | |
| ATP + [NFkappaB subunit p56] | Homo sapiens | - |
ADP + [NFkappaB subunit p56]phosphate | - |
? | |
| additional information | Homo sapiens | IKK activates and regulates NFkappaB important in intracellular signalling, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged IKKbeta from Sf21 insect cells by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HEK-293 cell | - |
Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
| JURKAT cell | - |
Homo sapiens | - |
| RAW-264.7 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [IkappaB protein] | - |
Homo sapiens | ADP + [IkappaB phosphoprotein] | - |
? | |
| ATP + [NFkappaB subunit p56] | - |
Homo sapiens | ADP + [NFkappaB subunit p56]phosphate | - |
? | |
| ATP + [NFkappaB subunit p56] | phosphorylation at serine residues, especially at S536, in the cytoplasm prior to NFkappaB p56 translocation to the nucleus | Homo sapiens | ADP + [NFkappaB subunit p56]phosphate | - |
? | |
| additional information | IKK activates and regulates NFkappaB important in intracellular signalling, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IKKbeta | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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