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Literature summary for 2.7.11.1 extracted from
- Arabidopsis casein kinase 2 alpha4 subunit regulates various developmental pathways in a functionally overlapping manner (2015), Plant Sci., 236, 295-303 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CKA4 or At2g23070, recombinant overexpression of GFP-tagged subunit CK2 alpha4 in Arabidopsis thaliana via Agrobacterium tumefaciens mediated transformation | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme knockout by construction of two independent CK2 alpha4 RNAi lines in the CK2 alpha triple mutant background, phenotype, overview. The RNAi lines in the wild-type background show 70-80% relative kinase activity while the relative kinase activity in the RNAi lines in CK2 alpha triple mutant background is reduced to 40-50%. Although the RNAi lines show up to 70-75% reduction inthe transcript level it does not correlate with the reduction seen in the relative kinase activity of the plant extracts. It islikely that the RNAi lines with severe reduction in kinase activity may not be viable and hence are not obtained from the screen | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | GFP-tagged CK2 alpha4 localizes to the chloroplast in transgenic Arabidopsis seedlings, consistent with the presence of a chloroplast localization signal at the N-terminus of CK2 alpha4 subunit. Subunit CK2 alpha4 also contains the internal conserved nuclear localization signal, NLS | Arabidopsis thaliana | 9507 | - |
| nucleus | in Arabidopsis thaliana, both alpha and beta subunits are redundantly encoded by four genes each and are ubiquitously expressed spatially and developmentally. All the CK2 alpha subunits except alpha4 are nuclear localized. Subunit CK2 alpha4 has an N-terminal chloroplast localization signal and also contains the internal conserved nuclear localization signal, NLS | Arabidopsis thaliana | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Arabidopsis thaliana |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O64816 | - |
- |
| Arabidopsis thaliana Col-0 | O64816 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + WheIF4B protein | recombinant artificial substrate | Arabidopsis thaliana | ADP + phospho-WheIF4B protein | - |
? | |
| ATP + WheIF4B protein | recombinant artificial substrate | Arabidopsis thaliana Col-0 | ADP + phospho-WheIF4B protein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | three of the four CK2 alpha subunit types existing in Arabidopsis act in a functionally redundant manner to regulate various developmental pathways | Arabidopsis thaliana |
| tetramer | CK2 is a tetrameric protein composed of two dimers formed of a alpha (catalytic) and a beta (regulatory) subunit each | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| casein kinase 2 | - |
Arabidopsis thaliana |
| casein kinase 2 alpha4 | - |
Arabidopsis thaliana |
| CK2 | - |
Arabidopsis thaliana |
| Ser/Thr kinase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | both CK2 alpha4 single knockout and CK2 alpha3alpha4 double knockout mutants are hyposensitive to abscisic acid induced block of seed germination | Arabidopsis thaliana |
| physiological function | three of the four CK2 alpha subunit types existing in Arabidopsis act in a functionally redundant manner to regulate various developmental pathways. The fourth alpha subunit in Arabidopsis thaliana, CK2 alpha4, also functions redundantly in regulating abscisic acid response, lateral root formation and flowering time, functionally overlapping role for the CK2 alpha4 subunit in regulating various developmental processes in plants. CK2 alpha4 positively regulates the block of seed germination inpresence of abscisic acid. Arabidopsis, the nuclear CK2 alpha subunits contribute synergistically to the block of seedgermination in response to hormone abscisic acid and salt in aconcentration dependent manner | Arabidopsis thaliana |
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