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Literature summary for 2.7.11.1 extracted from
- Casein kinase 2 phosphorylation of protein kinase C and casein kinase 2 substrate in neurons (PACSIN) 1 protein regulates neuronal spine formation (2013), J. Biol. Chem., 288, 9303-9312 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| brain-derived neurotrophic factor | BDNF | Rattus norvegicus | |
| brain-derived neurotrophic factor | BDNF | Mus musculus | |
| inositol 6-phosphate | specifically induces PACSIN 1 phosphorylation | Rattus norvegicus |  |
| inositol 6-phosphate | specifically induces PACSIN 1 phosphorylation | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Rattus norvegicus | 5737 | - |
| cytoplasm | - |
Mus musculus | 5737 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
| membrane | - |
Mus musculus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | required | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + PACSIN 1 protein | Rattus norvegicus | i.e. casein kinase 2 substrate in neurons 1 protein, casein kinase 2 phosphorylates PACSIN 1 at Ser358, no activity with PACSIN1 mutant S358A. Ser358 is located within the unstructured linker sequence connecting the N-terminal F-BAR domain with the C-terminal SH3 domain | ADP + phospho-PACSIN 1 protein | - |
? | |
| ATP + PACSIN 1 protein | Mus musculus | i.e. casein kinase 2 substrate in neurons 1 protein, casein kinase 2 phosphorylates PACSIN 1 at Ser358, no activity with PACSIN1 mutant S358A. Ser358 is located within the unstructured linker sequence connecting the N-terminal F-BAR domain with the C-terminal SH3 domain | ADP + phospho-PACSIN 1 protein | - |
? | |
| ATP + protein kinase C | Rattus norvegicus | - |
ADP + phospho-protein kinase C | - |
? | |
| ATP + protein kinase C | Mus musculus | - |
ADP + phospho-protein kinase C | - |
? | |
| additional information | Rattus norvegicus | dynamin is not phosphorylated in the cytosol, and SNAP-47 is not phosphorylated at all | ? | - |
? | |
| additional information | Mus musculus | dynamin is not phosphorylated in the cytosol, and SNAP-47 is not phosphorylated at all | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | O54833 | - |
- |
| Rattus norvegicus | P19139 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Rattus norvegicus | - |
| brain | - |
Mus musculus | - |
| hippocampus | - |
Rattus norvegicus | - |
| hippocampus | - |
Mus musculus | - |
| neuron | primary hippocampal neurons | Rattus norvegicus | - |
| neuron | primary hippocampal neurons | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + PACSIN 1 protein | i.e. casein kinase 2 substrate in neurons 1 protein, casein kinase 2 phosphorylates PACSIN 1 at Ser358, no activity with PACSIN1 mutant S358A. Ser358 is located within the unstructured linker sequence connecting the N-terminal F-BAR domain with the C-terminal SH3 domain | Rattus norvegicus | ADP + phospho-PACSIN 1 protein | - |
? | |
| ATP + PACSIN 1 protein | i.e. casein kinase 2 substrate in neurons 1 protein, casein kinase 2 phosphorylates PACSIN 1 at Ser358, no activity with PACSIN1 mutant S358A. Ser358 is located within the unstructured linker sequence connecting the N-terminal F-BAR domain with the C-terminal SH3 domain | Mus musculus | ADP + phospho-PACSIN 1 protein | - |
? | |
| ATP + protein kinase C | - |
Rattus norvegicus | ADP + phospho-protein kinase C | - |
? | |
| ATP + protein kinase C | - |
Mus musculus | ADP + phospho-protein kinase C | - |
? | |
| additional information | dynamin is not phosphorylated in the cytosol, and SNAP-47 is not phosphorylated at all | Rattus norvegicus | ? | - |
? | |
| additional information | dynamin is not phosphorylated in the cytosol, and SNAP-47 is not phosphorylated at all | Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| casein kinase 2 | - |
Rattus norvegicus |
| casein kinase 2 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
| 30 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rattus norvegicus |
| 7.5 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Rattus norvegicus | |
| ATP | - |
Mus musculus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | CK2-mediated phosphorylation regulates PACSIN 1 function, in vivo relevance of PACSIN 1 phosphorylation in neurons, overview. The majority of vesicle-associated, but not cytosolic, PACSIN 1 is enriched in the phosphoprotein fractions. PACSIN 1 Llpid association is not affected by Ser358 phosphorylation. CK2 phosphorylation of PACSIN 1 leads to a dissociation of the complex upon brain-derived neurotrophic factor, BDNF, treatment and induces Rac1-dependent spine formation in dendrites of hippocampal neurons. Upon BDNF signaling PACSIN 1 is phosphorylated by CK2 which is essential for spine formation. PACSIN 1 phosphorylation disrupts the PACSIN 1-NADRIN-Rac1 complex, which is essential for Rac1 hydrolysis and may explain the loss of spines in the presence of the PACSIN 1 mutant S358A | Rattus norvegicus |
| physiological function | CK2-mediated phosphorylation regulates PACSIN 1 function, in vivo relevance of PACSIN 1 phosphorylation in neurons, overview. The majority of vesicle-associated, but not cytosolic, PACSIN 1 is enriched in the phosphoprotein fractions. PACSIN 1 Llpid association is not affected by Ser358 phosphorylation. CK2 phosphorylation of PACSIN 1 leads to a dissociation of the complex upon brain-derived neurotrophic factor, BDNF, treatment and induces Rac1-dependent spine formation in dendrites of hippocampal neurons. Upon BDNF signaling PACSIN 1 is phosphorylated by CK2 which is essential for spine formation. PACSIN 1 phosphorylation disrupts the PACSIN 1-NADRIN-Rac1 complex, which is essential for Rac1 hydrolysis and may explain the loss of spines in the presence of the PACSIN 1 mutant S358A | Mus musculus |
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