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Literature summary for 2.7.11.1 extracted from
- Expression and characterization of Streptomyces coelicolor serine/threonine protein kinase PkaE (2015), Biosci. Biotechnol. Biochem., 79, 855-862.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces coelicolor |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Streptomyces coelicolor | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55600 | - |
- |
Streptomyces coelicolor |
| 80000 | - |
- |
Streptomyces coelicolor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + KbpA protein | Streptomyces coelicolor | - |
ADP + phosphorylated KbpA protein | - |
? |  |
| ATP + KbpA protein | Streptomyces coelicolor A3 (2) M145 | - |
ADP + phosphorylated KbpA protein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces coelicolor | Q9ZNB6 | - |
- |
| Streptomyces coelicolor A3 (2) M145 | Q9ZNB6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose column chromatography, and gel filtration | Streptomyces coelicolor |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| the recombinant enzyme is refolded into active forms by stepwise dialysis against buffer containing 50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 1 mM EDTA, and 1 mM dithiothreitol with decreasing urea concentration of 4, 2, and 1 M at 4 °C for 1 h | Streptomyces coelicolor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + KbpA protein | - |
Streptomyces coelicolor | ADP + phosphorylated KbpA protein | - |
? | |
| ATP + KbpA protein | - |
Streptomyces coelicolor A3 (2) M145 | ADP + phosphorylated KbpA protein | - |
? | |
| additional information | the recombinant enzyme is autophosphorylated at threonine residues only | Streptomyces coelicolor | ? | - |
? | |
| additional information | the recombinant enzyme is autophosphorylated at threonine residues only | Streptomyces coelicolor A3 (2) M145 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55600, calculated from amino acid sequence | Streptomyces coelicolor |
| ? | x * 80000, GST-tagged enzyme, SDS-PAGE | Streptomyces coelicolor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PkaE | - |
Streptomyces coelicolor |
| serine/threonine protein kinase | - |
Streptomyces coelicolor |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Streptomyces coelicolor | calculated from amino acid sequence | - |
6.2 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme acts as a negative regulator for blue pigment production | Streptomyces coelicolor |
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Release 2023.1 (January 2023)
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