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Literature summary for 2.7.11.1 extracted from

  • Maloverjan, A.; Piirsoo, M.; Michelson, P.; Kogerman, P.; Osterlund, T.
    Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway (2010), Exp. Cell Res., 316, 627-637.
    View publication on PubMed
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
gene ULK3, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of N-terminally FLAG-tagged wild-type and mutant enzymes in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K139R site-directed mutagenesis, the mutant is defective in autophosphorylation Homo sapiens
K44R site-directed mutagenesis, the mutant is defective in autophosphorylation Homo sapiens
additional information generation of a chimeric enzyme with an N-terminus of the mouse Shh enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6PHR2 gene ULK3
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein ULK3 is an autophosphorylated kinase Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally FLAG-tagged wild-type and mutant enzymes from HEK-293 cells by immunoaffinity precipitation Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland moderate ULK3 expression level Homo sapiens
-
brain high ULK3 expression level, highest in fetal brain, in all brain tissues, e.g. pons medulla, cortex, thalamus, hippocampus, cerebellum Homo sapiens
-
cerebellum
-
 Homo sapiens
-
colon
-
 Homo sapiens
-
heart very low ULK3 expression level Homo sapiens
-
hippocampus very high ULK3 expression level Homo sapiens
-
kidney high ULK3 expression level Homo sapiens
-
liver high ULK3 expression level Homo sapiens
-
lung low ULK3 expression level Homo sapiens
-
additional information ULK3 mRNA is widely expressed in humans with the highest expression in fetal brain and in a number of postnatal tissues where SHH signaling is active, tissue expression analysis, overview Homo sapiens
-
muscle
-
 Homo sapiens
-
olfactory bulb
-
 Homo sapiens
-
optic nerve
-
 Homo sapiens
-
pancreas
-
 Homo sapiens
-
placenta low ULK3 expression level Homo sapiens
-
prostate low ULK3 expression level Homo sapiens
-
salivary gland
-
 Homo sapiens
-
spinal cord
-
 Homo sapiens
-
spleen
-
 Homo sapiens
-
stomach low ULK3 expression level Homo sapiens
-
testis moderate ULK3 expression level Homo sapiens
-
thymus low ULK3 expression level Homo sapiens
-
trachea
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Gli1 protein low activity Homo sapiens ADP + phosphorylated Gli1 protein
-
 ?
ATP + Gli2 protein high activity Homo sapiens ADP + phosphorylated Gli2 protein
-
 ?
ATP + Gli3 protein low activity Homo sapiens ADP + phosphorylated Gli3 protein
-
 ?
additional information ULK3 shows autophosphorylation activity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
serine/threonine kinase
-
 Homo sapiens
ULK3
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
evolution human serine/threonine kinase ULK3 is annotated as belonging to unc-51-like family of serine/threonine kinases, but shares similarity with STK36 and dFu proteins Homo sapiens
malfunction kinase-deficient mutants of ULK3 are inactive in regulation of GLI protein activity Homo sapiens
physiological function the serine/threonine kinase ULK3 acts as a positive regulator of Hedgehog pathway and Gli proteins, ULK3 catalytical activity is crucial for its function in SHH pathway, overview. The Hedgehog signaling pathway plays crucial roles in embryonic development and is implicated in tissue homeostasis maintenance and neurogenesis in adults. ULK3 enhances endogenous and overexpressed GLI1 and GLI2 transcriptional activity in cultured cells, and ULK3 kinase activity is required for nuclear translocation of GLI1 from cytosol Homo sapiens