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Literature summary for 2.7.11.1 extracted from

  • Donat, S.; Streker, K.; Schirmeister, T.; Rakette, S.; Stehle, T.; Liebeke, M.; Lalk, M.; Ohlsen, K.
    Transcriptome and functional analysis of the eukaryotic-type serine/threonine kinase PknB in Staphylococcus aureus (2009), J. Bacteriol., 191, 4056-4069.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ 10 mM Mn2+ inhibits phosphorylation of myelin basic protein by PknB Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal kinase activity is detected in the presence of up to 5-10 mM Mg2+ Staphylococcus aureus
Mn2+ preferred cofactor, maximal kinase activity is detected in the presence of up to 5 mM Mn2+ Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 SDS-PAGE Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus 8325
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-TED column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenylosuccinate synthase PknB specifically phosphorylates adenylosuccinate synthase, phosphorylated adenylosuccinate synthase shows a 1.8fold decrease in enzymatic activity compared to unphosphorylated enzyme Staphylococcus aureus ADP + phosphorylated adenylosuccinate synthase
-
 ?
ATP + adenylosuccinate synthase PknB specifically phosphorylates adenylosuccinate synthase, phosphorylated adenylosuccinate synthase shows a 1.8fold decrease in enzymatic activity compared to unphosphorylated enzyme Staphylococcus aureus 8325 ADP + phosphorylated adenylosuccinate synthase
-
 ?
ATP + myelin basic protein
-
 Staphylococcus aureus ADP + phosphorylated myelin basic protein
-
 ?
ATP + myelin basic protein
-
 Staphylococcus aureus 8325 ADP + phosphorylated myelin basic protein
-
 ?

Synonyms

Synonyms Comment Organism
PknB
-
 Staphylococcus aureus
StpK
-
 Staphylococcus aureus

General Information

General Information Comment Organism
malfunction a pknB deletion strain showed 32-fold-higher sensitivity to tunicamycin Staphylococcus aureus