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Literature summary for 2.7.11.1 extracted from

  • Haraga, A.; Miller, S.I.
    A Salmonella type III secretion effector interacts with the mammalian serine/threonine protein kinase PKN1 (2006), Cell. Microbiol., 8, 837-846.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fatty acids activate PKN1 Homo sapiens
Rho activates PKN1 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of FLAG-tagged full-length PKN1 and of its isolated catalytic kinase domain, residues 561-942, in CHO-K1 cells, co-expression with Salmonella enterica SspH1, SspH2, YopM and IpaH9.8, all HA-tagged, in HeLa cells, interaction study, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
K644E site-directed mutagenesis, inactive mutant, PKN1 depletion causes an increase in NF-kappaB-dependent reporter gene expression Homo sapiens
additional information PKN1 depletion by siRNA causes an increase in NF-kappaB-dependent reporter gene expression Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme inhibits NF-kappaB activity via interaction with the leucine-rich repeat domain of the Salmonella enterica serovar typhimurium-derived effector SspH1 in infected intestine-407 cells, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation at Thr774 of the activation loop is required for activity in vivo, PKN1 is phosphorylated during Salmonella enterica infection independent of SspH1 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
intestine-407 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme inhibits NF-kappaB activity via interaction with the leucine-rich repeat domain of the Salmonella enterica serovar typhimurium-derived effector SspH1 in infected intestine-407 cells, overview Homo sapiens ?
-
?
additional information the catalytic kinase domain, residues 561-942, of PKN1 is active with out the regulatory domain Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
PKN1
-
Homo sapiens
protein kinase N1
-
Homo sapiens
serine/threonine protein kinase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens