Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | RSK1 stimulation by EGF via CREB, a cAMP-response element-binding protein, autophosphorylation at Ser363 and Ser380 of RSK1 and membrane translocation are required for full RSK1 activation | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
in vitro transcription and translation of RSK1, expression of RSK1 in PS127A cells or in HEK-293 cells, coexpression and colocalization in cytoplasm of FLAG-tagged RSK1 and 14-3-3beta in COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S154A | site-directed mutagenesis, mutation modulates the enzyme activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
14-3-3beta | a p90 ribosomal S6 kinase isoform 1-binding protein that negatively regulates RSK1 kinase activity, interaction via Lys49 of 14-3-3beta and S154 of phosphorylated RSK1, phosphorylation of RSK1 is required for interaction, no binding of mutant 14-3-3beta K49Q or K49A | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
plasma membrane | membrane translocation is required for full RSK1 activation | Rattus norvegicus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus | |
Mn2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | isozyme RSK1, i.e. 14-3-3, is regulated by inhibitory binding of 14-3-3beta, a RSK1-binding protein, to Lys49 of the enzyme, Lys49 is also responsible for interaction of RSK1 with Bcr, Raf, and Cbl | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
isozyme RSK1, i.e. p90 ribosomal S6 kinase or 14-3-3 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | RSK1 is autophosphorylated at Ser363 and Ser380 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isozyme RSK1, i.e. 14-3-3, is regulated by inhibitory binding of 14-3-3beta, a RSK1-binding protein, to Lys49 of the enzyme, Lys49 is also responsible for interaction of RSK1 with Bcr, Raf, and Cbl | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
14-3-3 | - |
Rattus norvegicus |
p90 ribosomal S6 kinase | - |
Rattus norvegicus |
RSK | - |
Rattus norvegicus |
RSK1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |