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Literature summary for 2.7.11.1 extracted from

  • Sharma, K.; Chandra, H.; Gupta, P.K.; Pathak, M.; Narayan, A.; Meena, L.S.; D'Souza, R.C.J.; Chopra, P.; Ramachandran, S.; Singh, Y.
    PknH, a transmembrane Hank's type serine/threonine kinase from Mycobacterium tuberculosis is differentially expressed under stress conditions (2004), FEMS Microbiol. Lett., 233, 107-113.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Rv1266c or pknH, overexpression of wild-type and mutant GST-fusion PknH in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
K45M site-directed mutagenesis, inactive mutant Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
H-7
-
Mycobacterium tuberculosis
additional information enzyme expression level is decreased under low pH and heat shock conditions Mycobacterium tuberculosis
staurosporine
-
Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane PknH is a transmembrane protein Mycobacterium tuberculosis 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+, dependent on Mycobacterium tuberculosis
Mn2+ Mg2+ or Mn2+, dependent on Mycobacterium tuberculosis
additional information no activity with cations other than Mg2+ or Mn2+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE Mycobacterium tuberculosis
97000
-
x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycobacterium tuberculosis enzyme is involved in stress response and adaptation to environmental changes in mycobacteria ?
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
strains H37Rv and H37Ra, PknH is specific for mycobacteria of the Mycobacterium tuberculosis complex
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein PknH performs autophosphorylation, mutant K45M is inactive Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant GST-fusion PknH from Escherichia coli by glutathione affinity chromatography Mycobacterium tuberculosis

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate Lys45 is important for PknH catalytic and autophosphorylation activity Mycobacterium tuberculosis

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme expression level under stress conditions, overview Mycobacterium tuberculosis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone
-
Mycobacterium tuberculosis ADP + phosphorylated histone
-
?
ATP + myelin basic protein
-
Mycobacterium tuberculosis ADP + phosphorylated myelin basic protein
-
?
additional information enzyme is involved in stress response and adaptation to environmental changes in mycobacteria Mycobacterium tuberculosis ?
-
?
additional information PknH performs autophosphorylation Mycobacterium tuberculosis ?
-
?

Subunits

Subunits Comment Organism
? x * 97000, recombinant GST-fusion PknH, SDS-PAGE, x * 68000, non-tagged PknH, SDS-PAGE Mycobacterium tuberculosis
More PknH contains 11 subdomains, schematic structure, overview Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
Hank's type serine/threonine kinase
-
Mycobacterium tuberculosis
PknH
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mycobacterium tuberculosis

pI Value

Organism Comment pI Value Maximum pI Value
Mycobacterium tuberculosis amino acid sequence calculation
-
6.3