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Literature summary for 2.7.11.1 extracted from

  • Bucko-Justyna, M.; Lipinski, L.; Burgering, B.M.; Trzeciak, L.
    Characterization of testis-specific serine-threonine kinase 3 and its activation by phosphoinositide-dependent kinase-1-dependent signalling (2005), FEBS J., 272, 6310-6323.
    View publication on PubMed
Search for more literature for 2.7.11.1

Activating Compound

Activating Compound Comment Organism Structure
additional information insulin induces the phosphorylation of TSSK3, TSSK3 activation by phosphoinositide-dependent kinase-1 PDK1-dependent signalling, phosphorylation at Thr168 activates Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant GST-fusion TSSK3s in Escherichia coli strain BL21, expression of Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 in human 293T cells Mus musculus

Protein Variants

Protein Variants Comment Organism
K39R site-directed mutagenesis, inactive mutant Mus musculus
S166A site-directed mutagenesis, mutation of a T-loop residue, highly reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation Mus musculus
S166D site-directed mutagenesis, mutation of a T-loop residue, reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation Mus musculus
S166G site-directed mutagenesis, mutation of a T-loop residue, highly reduced kinase activity compared to the wild-type TSSK3, no autophosphorylation Mus musculus
T168A site-directed mutagenesis, mutation of a T-loop residue, inactive TSSK3 mutant, no autophosphorylation Mus musculus
T168D site-directed mutagenesis, mutation of a T-loop residue, unaltered kinase activity and reduced autophosphorylation compared to the wild-type TSSK3 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.145
-
 myelin basic protein pH 7.4, 30°C, recombinant TSSK3 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus
Mn2+ preferred cation, optimal at 10 mM Mus musculus
additional information divalent cations are required for activity Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + testis-specific serine-threonine kinase 3 Mus musculus substrate of phosphoinositide-dependent kinase-1 leading to activation ADP + phosphorylated testis-specific serine-threonine kinase 3
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein TSSK3 performs autophosphorylation, TSSK3 is phosphorylated at Thr168 of the T-loop by recombinant Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 leading to activation of TSSK3, TSSK3 can also be phosphorylated by cAMP-dependent protein kinase PKA, EC 2.7.11.11, but not by protein kinase B Mus musculus

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant GST-fusion TSSK3s by glutathione affinity chromatography, recombinant Myc-tagged catalytic subunit of phosphoinositide-dependent kinase-1 from human 293T cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
testis testis-specific serine-threonine kinase 3, i.e. TSSK3 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein substrate of TSSK3 Mus musculus ADP + phosphorylated casein
-
 ?
ATP + histone H1 substrate of TSSK3 Mus musculus ADP + phosphorylated histone H1
-
 ?
ATP + myelin basic protein substrate of TSSK3 Mus musculus ADP + phosphorylated myelin basic protein
-
 ?
ATP + RRSSSY peptide substrate of TSSK3 consisting of the consensus sequence Mus musculus ADP + RRSS-phosphoS-Y
-
 ?
ATP + testis-specific serine-threonine kinase 3 substrate of phosphoinositide-dependent kinase-1 leading to activation Mus musculus ADP + phosphorylated testis-specific serine-threonine kinase 3
-
 ?
ATP + testis-specific serine-threonine kinase 3 substrate of recombinant Myc-tagged catalytic subunit of PDK1 leading to activation Mus musculus ADP + phosphorylated testis-specific serine-threonine kinase 3
-
 ?
additional information TSSK3 performs autophosphorylation Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
More TSSK3 contains a T-loop structure Mus musculus

Synonyms

Synonyms Comment Organism
More the enzyme TSSK3 belongs to the family of testis-specific serine-threonine kinases Mus musculus
PDK1
-
 Mus musculus
phosphoinositide-dependent kinase-1
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 TSSK3, and phosphoinositide-dependent kinase-1 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 TSSK3 Mus musculus
7.5
-
 PDK1 assay at Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.2 TSSK3 shows a broad range pH-optimum Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on, cannot be substituted by other nucleotide triphosphates like CTP or GTP Mus musculus