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Literature summary for 2.7.11.1 extracted from

  • Novakova, L.; Saskova, L.; Pallova, P.; Janecek, J.; Novotna, J.; Ulrych, A.; Echenique, J.; Trombe, M.C.; Branny, P.
    Characterization of a eukaryotic type serine/threonine protein kinase and protein phosphatase of Streptococcus pneumoniae and identification of kinase substrates (2005), FEBS J., 272, 1243-1254.
    View publication on PubMed
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged StkP and of GST-fusion StkP in Escherichia coli strain BL21(DE3) Streptococcus pneumoniae

Protein Variants

Protein Variants Comment Organism
K42R site-directed mutagenesis, mutant StkP activity is reduced by 87% compared to the wild-type enzyme Streptococcus pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
staurosporine
-
 Streptococcus pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Streptococcus pneumoniae 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Streptococcus pneumoniae
Mn2+ preferred cation, StkP Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Streptococcus pneumoniae protein kinase StkP and Mn2+-dependent cytosolic protein phosphatase PhpP act as a functional pair in vivo ?
-
 ?
additional information Streptococcus pneumoniae Cp 1015 protein kinase StkP and Mn2+-dependent cytosolic protein phosphatase PhpP act as a functional pair in vivo ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae Cp 1015
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein StkP performs autophosphorylation Streptococcus pneumoniae

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion StkP from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the GST-tag is cleaved off by factor Xa, no binding of His-tagged recombinant enzyme to a nickel chelating matrix can be achieved Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phosphoglucosamine mutase
-
 Streptococcus pneumoniae ADP + phosphorylated phosphoglucosamine mutase
-
 ?
ATP + phosphoglucosamine mutase
-
 Streptococcus pneumoniae Cp 1015 ADP + phosphorylated phosphoglucosamine mutase
-
 ?
additional information protein kinase StkP and Mn2+-dependent cytosolic protein phosphatase PhpP act as a functional pair in vivo Streptococcus pneumoniae ?
-
 ?
additional information StkP performs autophosphorylation, StkP is dephosphorylated by PhpP, a PP2C-type protein phosphatase Streptococcus pneumoniae ?
-
 ?
additional information protein kinase StkP and Mn2+-dependent cytosolic protein phosphatase PhpP act as a functional pair in vivo Streptococcus pneumoniae Cp 1015 ?
-
 ?
additional information StkP performs autophosphorylation, StkP is dephosphorylated by PhpP, a PP2C-type protein phosphatase Streptococcus pneumoniae Cp 1015 ?
-
 ?

Synonyms

Synonyms Comment Organism
Ser/Thr protein kinase
-
 Streptococcus pneumoniae
serine/threonine protein kinase
-
 Streptococcus pneumoniae
StpK
-
 Streptococcus pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
3 9 StkP Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Streptococcus pneumoniae