Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.1 extracted from

  • Vitari, A.C.; Deak, M.; Morrice, N.A.; Alessi, D.R.
    The WNK1 and WNK4 protein kinases that are mutated in Gordon's hypertension syndrome phosphorylate and activate SPAK and OSR1 protein kinases (2005), Biochem. J., 391, 17-24.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of WNK1 and truncated WNK4 comprising residues 1-593 in HEK-293 cells, co-expression of truncated WNK1 comprising residues 1-661 with claudin-4, oxidative stress response kinase 1 OSR1, STE/SPS1-related proline/alanine-rich kinase SPAK, and the Na+-K+-2Cl- co-transporter-1-(1-260) construct NKCC1-(1-260) in Escherichia coli strain BL21 as GST-fusion proteins Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
WNK1 partially from testis, recombinant GST-fusion truncated WNK1 comprising residues 1-661 with claudin-4, wild-type and mutant oxidative stress response kinase 1 OSR1, STE/SPS1-related proline/alanine-rich kinase SPAK, and Na+-K+-2Cl- co-transporter-1-(1-260) construct NKCC1-(1-260) from Escherichia coli by glutathione affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue very low expression level of WNK1 Rattus norvegicus
-
brain very low expression level of WNK1 Rattus norvegicus
-
heart very low expression level of WNK1 Rattus norvegicus
-
liver very low expression level of WNK1 Rattus norvegicus
-
pancreas low expression level of WNK1 Rattus norvegicus
-
spleen very low expression level of WNK1 Rattus norvegicus
-
testis WNK4, high expression level of WNK1 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + claudin-4 recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli Rattus norvegicus ADP + phosphorylated claudin-4
-
 ?
ATP + myelin basic protein recombinant WNK1 or WNK4 expressed in Escherichia coli Rattus norvegicus ADP + phosphorylated myelin basic protein
-
 ?
ATP + NKCC1-(1-260) i.e. Na+-K+-2Cl- co-transporter-1-(1-260) construct, recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli Rattus norvegicus ADP + phosphorylated NKCC1-(1-260)
-
 ?
ATP + oxidative stress response kinase 1 OSR1 recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli, phosphorylation at Ser325 and Thr185, the latter is required for activation of OSR1 Rattus norvegicus ADP + phosphorylated oxidative stress response kinase 1 OSR1
-
 ?
ATP + STE/SPS1-related proline/alanine-rich kinase SPAK recombinant WNK1 or WNK4 with recombinant protein substrate, both expressed in Escherichia coli Rattus norvegicus ADP + phosphorylated STE/SPS1-related proline/alanine-rich kinase SPAK
-
 ?
additional information WNK1 interacts with OSR1 and SPAK, overview Rattus norvegicus ?
-
 ?

Synonyms

Synonyms Comment Organism
WNK1
-
 Rattus norvegicus
WNK4
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Rattus norvegicus