Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphorylation of Tyr397 in Lyn results in activation of the enzyme | Mus musculus |
Application | Comment | Organism |
---|---|---|
medicine | Lyn kinase inhibitors are becoming an increasingly important class of chemotherapeutic agents | Mus musculus |
Cloned (Comment) | Organism |
---|---|
the murine Lyn kinase domain, amino acids 239-512, is subcloned into the baculovirus expression vector pFastBacHTA generating a His6-tagged fusion resulting in a 5 amino acid N-terminal extension to the Lyn kinase domain after tobacco etch virus cleavage | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of the kinase domain of Lyn are determined in complex with three different inhibitors, AMP-PNP, 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine and dasatinib, as well as the non-liganded form of the enzyme, at 2.5 to 2.7 A resolution | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine | PP2, AG-1897 | Mus musculus | |
AMP-PNP | - |
Mus musculus | |
dasatinib | - |
Mus musculus | |
additional information | phosphorylation of Tyr508 in Lyn inhibits activity of the enzyme | Mus musculus | |
SU6656 | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-L-tyrosine | Mus musculus | - |
ADP + [protein]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using nickel-chelating resin, a HiTrap Q column and gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Cbp | murine Csk binding protein, Cbp, amino acids 74-474, substrate used in kinase activity assay | Mus musculus | ? + ADP | - |
? | |
ATP + [protein]-L-tyrosine | - |
Mus musculus | ADP + [protein]-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Lyn protein tyrosine kinase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
kinase assay | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
kinase assay | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000009 | - |
substrate Csk binding protein, Cbp | Mus musculus | 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine | |
0.000011 | - |
substrate Csk binding protein, Cbp | Mus musculus | dasatinib | |
0.000035 | - |
substrate Csk binding protein, Cbp | Mus musculus | SU6656 |