Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | protein kinase activation through an intra-molecular autophosphorylation process | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
the Etk C-terminal kinase domain, 451-726, and Y574A, R614A mutants are cloned into pET vectors for expression in Escherichia coli BL21DE3 cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
the C-terminal kinase domain of Escherichia coli Tyr kinase Etk is solved at 2.5 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R614A | mutant, used in autokinase activity assay | Escherichia coli |
R614K | mutant, used in autokinase activity assay | Escherichia coli |
Y574A | mutant, used in autokinase activity assay | Escherichia coli |
Y574E | mutant, used in autokinase activity assay | Escherichia coli |
Y574F | mutant, used in autokinase activity assay | Escherichia coli |
Y574G | mutant, used in autokinase activity assay | Escherichia coli |
Y574N | mutant, used in autokinase activity assay | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Escherichia coli | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-L-tyrosine | Escherichia coli | - |
ADP + [protein]-L-tyrosine phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P58764 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Escherichia coli |
Purification (Comment) | Organism |
---|---|
by nickel nitrilotriacetate agarose affinity chromatography and gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild type activity 100%, mutant Y574A 98.4%, mutant Y574E 100%, mutant Y574F 12.1%, mutant Y574G 86.7%, mutant Y574N 26.9%, mutant R614A 14.6%, mutant R614K 61.8% | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-L-tyrosine | - |
Escherichia coli | ADP + [protein]-L-tyrosine phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrosine kinase Etk | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
kinase activity assay at room temperature | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
kinase activity assay | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |