Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation mechanism, autophosphorylation of Tyr551 causes a 10-fold increase in BTK activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y551F | site-directed mutagenesis, the mutant enzyme cannot be activated by autophosphorylation at Y551, and shows about 10fold reduced activation and activity compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive versus ATP, noncompetitive versus S1 peptide | Homo sapiens | |
staurosporine | competitive versus ATP, noncompetitive versus S1 peptide | Homo sapiens | |
tyrophostin A25 | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | BTK steady state kinetics, kinetic mechanism, wild-type BTK and mutant Y551F | Homo sapiens | |
0.037 | - |
S1 peptide | BTK | Homo sapiens | |
0.084 | 0.09 | ATP | preactivated wild-type BTK | Homo sapiens | |
0.106 | - |
ATP | untreated wild-type BTK | Homo sapiens | |
0.124 | - |
ATP | preactivated BTK mutant Y551 | Homo sapiens | |
17.64 | - |
ATP | untreated BTK mutant Y551 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | BTK rapidly autophosphorylates at Tyr551 within its activation loop in vitro, phosphorylation of Tyr551 causes a 10fold increase in BTK activity | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + F peptide | i.e. biotin-Aca-AAAEEIFGEI-NH2 | Homo sapiens | ADP + phospho-L-tyrosinyl-F peptide | - |
? | |
ATP + P1 peptide | i.e. biotin-Aca-AAAEEIpYGEI-NH2 | Homo sapiens | ADP + phospho-L-tyrosinyl-P1 peptide | - |
? | |
ATP + S1 peptide | i.e. biotin-Aca-AAAEEIYGEI-NH2 | Homo sapiens | ADP + phospho-L-tyrosinyl-S1 peptide | - |
? | |
additional information | BTK performs autophosphorylation | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Brutons tyrosine kinase | - |
Homo sapiens |
Btk | - |
Homo sapiens |
More | the enzyme belongs to the Tec nonreceptor tyrosine kinase family | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate inhibition kinetics and analysis | Homo sapiens |