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Literature summary for 2.7.10.2 extracted from
- Conserved hydrophobicity in the SH2-kinase linker is required for catalytic activity of Csk and CHK (2003), FEBS Lett., 544, 11-14.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Csk and CHK require conserved hydrophobicity in the SH2-kinase linker for catalytic activity | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression of Csk in HeLa cells, overexpression of FLAG-tagged CHK in COS-1 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F183A | site-directed mutagenesis, about 70% reduced Csk tyrosine kinase activity compared to the wild-type enzyme | Homo sapiens |
| F183L | site-directed mutagenesis, about 40% reduced Csk tyrosine kinase activity compared to the wild-type enzyme | Homo sapiens |
| F183W | site-directed mutagenesis, about 50% reduced Csk tyrosine kinase activity compared to the wild-type enzyme | Homo sapiens |
| K222R | site-directed mutagenesis, about 80% reduced Csk tyrosine kinase activity compared to the wild-type enzyme | Homo sapiens |
| L223A | site-directed mutagenesis, about 40% reduced Csk tyrosine kinase activity compared to the wild-type enzyme | Homo sapiens |
| L223F | site-directed mutagenesis, only slightly reduced Csk tyrosine kinase activity compared to the wild-type enzyme | Homo sapiens |
| additional information | deletion of SH2-kinase linker residues V177-Y184 reduces the catalytic activity of Csk to 20% of wild-type enzyme activity | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification of recombinant Csk and FLAG-tagged CHK by specific immunoprecipitation | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + poly(Glu4-Tyr) | - |
Homo sapiens | ADP + poly(Glu4-Tyr)-L-tyrosine phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | Csk and CHK require conserved hydrophobicity in the SH2-kinase linker for catalytic activity | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C-terminal Src kinase | - |
Homo sapiens |
| CHK | - |
Homo sapiens |
| Csk | - |
Homo sapiens |
| Csk homologous kinase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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