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Literature summary for 2.7.10.1 extracted from

  • Schmidt-Arras, D.E.; Bohmer, A.; Markova, B.; Choudhary, C.; Serve, H.; Bohmer, F.D.
    Tyrosine phosphorylation regulates maturation of receptor tyrosine kinases (2005), Mol. Cell. Biol., 25, 3690-3703.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type FLT-3 and mutant FLT-3 ITD in HEK293 cells, expression of hemagglutinin-tagged wild-type FLT-3, containing an ER-targeting sequence, in THP-1 cells, expression of hemagglutinin-tagged or EGFP-tagged, connected via a six-glycine-linker, FLT-3 in COS-7 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
K634A site-directed mutagenesis PDGFbeta receptor tyrosine kinase, results in impaired tyrosine autophosphorylation and maturation, as well as loss of kinase activity Homo sapiens
K644A site-directed mutagenesis of juxtamembrane tandem mutagenic construct of FLT-3 RTK and of wild-type FLT-3 RTK, maturation of the mutants by glycosylation and phosphorylation is impaired, altered localization compaired to the wild-type enzyme, overview Homo sapiens
additional information construction of FLT-3 with a tandem repeat of the juxtamembrane, termed FLT-3 ITD RTK, showing impaired maturation during recombinant expression in MV4-11 cells, the mutation alters enzyme interactions with other proteins, e.g. heat shock proteins, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
AG1296 FLT-3 inhibitor treatment results in formation of larger amounts of the mature 150 kDa form of mutant FLT-3 ITD Homo sapiens
SU11248 FLT-3 inhibitor treatment results in formation of larger amounts of the mature 150 kDa form of mutant FLT-3 ITD Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE Homo sapiens
150000
-
x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a [protein]-L-tyrosine Homo sapiens FLT-3 is involved in signal transduction, mechanisms ADP + a [protein]-L-tyrosine phosphate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein FLT-3 and FLT-3 ITD are high-mannose-type glycoproteins Homo sapiens
phosphoprotein tyrosine autophosphorylation regulates maturation of the FLT-3 RTK and of PDGFbeta receptor Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a [protein]-L-tyrosine
-
Homo sapiens ADP + a [protein]-L-tyrosine phosphate
-
?
ATP + a [protein]-L-tyrosine FLT-3 is involved in signal transduction, mechanisms Homo sapiens ADP + a [protein]-L-tyrosine phosphate
-
?

Subunits

Subunits Comment Organism
? x * 130000, recombinant FLT-3 ITD mutant, SDS-PAGE, x * 150000, recombinant wild-type FLT-3, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
FLT-3
-
Homo sapiens
fms-like tyrosine kinase 3
-
Homo sapiens
receptor tyrosine kinase
-
Homo sapiens
RTK
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens