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Literature summary for 2.7.1.B29 extracted from

  • Motoyama, K.; Sobue, F.; Kawaide, H.; Yoshimura, T.; Hemmi, H.
    Conversion of mevalonate 3-kinase into 5-phosphomevalonate 3-kinase by single amino acid mutations (2019), Appl. Environ. Microbiol., 85, e00256-19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.B29

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged E140 mutants of mevalonate 3-kinase, functioning as 5-phosphomevalonate 3-kinases, in Escherichia coli strain BL21 Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
additional information substrate-interacting glutamate residue E140 of Thermoplasma acidophilum mevalonate 3-kinase (EC 2.7.1.185) is replaced by smaller amino acids, including its counterparts in diphosphomevalonate decarboxylase and phosphomevalonate decarboxylase, with the aim of altering substrate specificity. These single amino acid mutations results in the conversion of mevalonate 3-kinase into 5-phosphomevalonate 3-kinase, which can synthesize 3,5-bisphosphomevalonate from 5-phosphomevalonate. The mutants catalyzing the hitherto undiscovered reaction enables the construction of an artificial mevalonate pathway in Escherichia coli cells, as is demonstrated by the accumulation of lycopene, a red carotenoid pigment. Alternative MVA pathway II overview. Constructed plasmids and strains, overview Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-5-phosphomevalonate Thermoplasma acidophilum
-
 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate Thermoplasma acidophilum JCM 9062
-
 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate Thermoplasma acidophilum AMRC-C165
-
 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate Thermoplasma acidophilum ATCC 25905
-
 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate Thermoplasma acidophilum NBRC 15155
-
 ADP + (R)-3,5-bisphosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-
Thermoplasma acidophilum AMRC-C165
-
-
-
Thermoplasma acidophilum ATCC 25905
-
-
-
Thermoplasma acidophilum JCM 9062
-
-
-
Thermoplasma acidophilum NBRC 15155
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzymes from Escherichia coli strain BL21 by nickel affinity chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate
-
 Thermoplasma acidophilum ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate
-
 Thermoplasma acidophilum JCM 9062 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate
-
 Thermoplasma acidophilum AMRC-C165 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate
-
 Thermoplasma acidophilum ATCC 25905 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate
-
 Thermoplasma acidophilum NBRC 15155 ADP + (R)-3,5-bisphosphomevalonate
-
 ?
additional information substrate specificity analysis, no reactivity toward MVA 5-diphosphate. Electrospray ionization mass spectrometry (ESI-MS) analysis of the product is performed in the negative mode using the direct infusion method Thermoplasma acidophilum ?
-
-
additional information substrate specificity analysis, no reactivity toward MVA 5-diphosphate. Electrospray ionization mass spectrometry (ESI-MS) analysis of the product is performed in the negative mode using the direct infusion method Thermoplasma acidophilum JCM 9062 ?
-
-
additional information substrate specificity analysis, no reactivity toward MVA 5-diphosphate. Electrospray ionization mass spectrometry (ESI-MS) analysis of the product is performed in the negative mode using the direct infusion method Thermoplasma acidophilum AMRC-C165 ?
-
-
additional information substrate specificity analysis, no reactivity toward MVA 5-diphosphate. Electrospray ionization mass spectrometry (ESI-MS) analysis of the product is performed in the negative mode using the direct infusion method Thermoplasma acidophilum ATCC 25905 ?
-
-
additional information substrate specificity analysis, no reactivity toward MVA 5-diphosphate. Electrospray ionization mass spectrometry (ESI-MS) analysis of the product is performed in the negative mode using the direct infusion method Thermoplasma acidophilum NBRC 15155 ?
-
-

General Information

General Information Comment Organism
physiological function a substrate-interacting glutamate residue of Thermoplasma acidophilum mevalonate 3-kinase (EC 2.7.1.185) is replaced by smaller amino acids, including its counterparts in diphosphomevalonate decarboxylase and phosphomevalonate decarboxylase, with the aim of altering substrate specificity. These single amino acid mutations E140S and E140G result in the conversion of mevalonate 3-kinase into 5-phosphomevalonate 3-kinase, which can synthesize 3,5-bisphosphomevalonate from 5-phosphomevalonate. The mutants catalyzing the hitherto undiscovered reaction enables the construction of an artificial mevalonate pathway in Escherichia coli cells, as is demonstrated by the accumulation of lycopene, a red carotenoid pigment Thermoplasma acidophilum