BRENDA - Enzyme Database show
show all sequences of 2.7.1.B20

The phosphofructokinase-B (MJ0406) from Methanocaldococcus jannaschii represents a nucleoside kinase with a broad substrate specificity

Hansen, T.; Arnfors, L.; Ladenstein, R.; Schönheit, P.; Extremophiles 11, 105-114 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Methanocaldococcus jannaschii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
cytidine
pH 7.0, 50°C, cosubstrate: ATP
Methanocaldococcus jannaschii
0.021
-
Inosine
pH 7.0, 50°C, cosubstrate: ATP
Methanocaldococcus jannaschii
0.062
-
guanosine
pH 7.0, 70°C, cosubstrate: ATP
Methanocaldococcus jannaschii
0.078
-
guanosine
pH 7.0, 50°C, cosubstrate: ATP
Methanocaldococcus jannaschii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Mg2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Mn2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Ni2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 34000, SDS-PAGE
Methanocaldococcus jannaschii
68000
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Methanocaldococcus jannaschii
the enzyme plays a role in nucleotide metabolism
?
-
-
-
additional information
Methanocaldococcus jannaschii DSM 2661
the enzyme plays a role in nucleotide metabolism
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
Q57849
-
-
Methanocaldococcus jannaschii DSM 2661
Q57849
-
-
Purification (Commentary)
Commentary
Organism
-
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cytidine
at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii
ADP + CMP
-
-
-
?
ATP + cytidine
at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii DSM 2661
ADP + CMP
-
-
-
?
ATP + guanosine
at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii
ADP + GMP
-
-
-
?
ATP + guanosine
at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii DSM 2661
ADP + GMP
-
-
-
?
ATP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
ADP + IMP
-
-
-
?
ATP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii DSM 2661
ADP + IMP
-
-
-
?
GTP + cytidine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
GDP + CMP
-
-
-
?
GTP + guanosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
GDP + GMP
-
-
-
?
GTP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
GDP + IMP
-
-
-
?
ITP + cytidine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
IDP + CMP
-
-
-
?
ITP + guanosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
IDP + GMP
-
-
-
?
ITP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
IDP + IMP
-
-
-
?
additional information
the enzyme plays a role in nucleotide metabolism
727430
Methanocaldococcus jannaschii
?
-
-
-
-
additional information
adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate
727430
Methanocaldococcus jannaschii
?
-
-
-
-
additional information
the enzyme plays a role in nucleotide metabolism
727430
Methanocaldococcus jannaschii DSM 2661
?
-
-
-
-
additional information
adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate
727430
Methanocaldococcus jannaschii DSM 2661
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homodimer
2 * 34000, SDS-PAGE
Methanocaldococcus jannaschii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
-
Methanocaldococcus jannaschii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
90
-
melting temperature
Methanocaldococcus jannaschii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Methanocaldococcus jannaschii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.1
8.2
50% of maximal activity at pH 6.1 and at pH 8.2
Methanocaldococcus jannaschii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.017
-
cytidine
pH 7.0, 50°C, cosubstrate: ATP
Methanocaldococcus jannaschii
0.021
-
Inosine
pH 7.0, 50°C, cosubstrate: ATP
Methanocaldococcus jannaschii
0.062
-
guanosine
pH 7.0, 70°C, cosubstrate: ATP
Methanocaldococcus jannaschii
0.078
-
guanosine
pH 7.0, 50°C, cosubstrate: ATP
Methanocaldococcus jannaschii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Mg2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Mn2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Ni2+
enzyme activity requires the presence of divalent cations. Mg2+ (100%) can be efficiently replaced by Mn2+ (97%) and partially by Ni2+ (31%) or Co2+ (6%)
Methanocaldococcus jannaschii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 34000, SDS-PAGE
Methanocaldococcus jannaschii
68000
-
gel filtration
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Methanocaldococcus jannaschii
the enzyme plays a role in nucleotide metabolism
?
-
-
-
additional information
Methanocaldococcus jannaschii DSM 2661
the enzyme plays a role in nucleotide metabolism
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanocaldococcus jannaschii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + cytidine
at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii
ADP + CMP
-
-
-
?
ATP + cytidine
at 70°C the Vmax/Km-value for cytidine is 93% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for cytidine is 59% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii DSM 2661
ADP + CMP
-
-
-
?
ATP + guanosine
at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii
ADP + GMP
-
-
-
?
ATP + guanosine
at 70°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. At 50°C the Vmax/Km-value for guanosine is 52% of the Vmax/Km-value for inosine. Neither ADP, acetyl phosphate, nor diphosphate can replace ATP
727430
Methanocaldococcus jannaschii DSM 2661
ADP + GMP
-
-
-
?
ATP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
ADP + IMP
-
-
-
?
ATP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii DSM 2661
ADP + IMP
-
-
-
?
GTP + cytidine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
GDP + CMP
-
-
-
?
GTP + guanosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
GDP + GMP
-
-
-
?
GTP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
GDP + IMP
-
-
-
?
ITP + cytidine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
IDP + CMP
-
-
-
?
ITP + guanosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
IDP + GMP
-
-
-
?
ITP + inosine
relative phosphoryl donor specificity: ATP (100%), ITP (41%), GTP (40%), UTP (8%), CTP (6%)
727430
Methanocaldococcus jannaschii
IDP + IMP
-
-
-
?
additional information
the enzyme plays a role in nucleotide metabolism
727430
Methanocaldococcus jannaschii
?
-
-
-
-
additional information
adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate
727430
Methanocaldococcus jannaschii
?
-
-
-
-
additional information
the enzyme plays a role in nucleotide metabolism
727430
Methanocaldococcus jannaschii DSM 2661
?
-
-
-
-
additional information
adenosine, xanthosine, 2'-deoxyadenosine, uridine, and thymidine are used at low rates, the enzyme catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate at an extremely low rate
727430
Methanocaldococcus jannaschii DSM 2661
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 34000, SDS-PAGE
Methanocaldococcus jannaschii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
-
-
Methanocaldococcus jannaschii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
90
-
melting temperature
Methanocaldococcus jannaschii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Methanocaldococcus jannaschii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.1
8.2
50% of maximal activity at pH 6.1 and at pH 8.2
Methanocaldococcus jannaschii
General Information
General Information
Commentary
Organism
physiological function
the enzyme plays a role in nucleotide metabolism
Methanocaldococcus jannaschii
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme plays a role in nucleotide metabolism
Methanocaldococcus jannaschii
Other publictions for EC 2.7.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728780
Elkin
A broad specificity nucleoside ...
Thermoplasma acidophilum
Proteins
81
568-582
2013
-
-
1
1
-
-
-
6
1
3
2
-
-
4
-
-
1
-
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
6
1
3
2
-
-
-
-
1
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
6
6
697882
Ota
A novel nucleoside kinase from ...
Burkholderia thailandensis, Burkholderia thailandensis DSM 13276
FEBS J.
275
5865-5872
2008
-
-
1
-
-
-
2
8
-
3
2
-
-
7
-
-
1
-
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
8
-
3
2
-
-
-
-
1
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
8
8
726575
Arnfors
Structure of Methanocaldococcu ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Acta Crystallogr. Sect. D
62
1085-1097
2006
-
-
-
1
-
-
-
-
-
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727430
Hansen
The phosphofructokinase-B (MJ0 ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Extremophiles
11
105-114
2006
-
-
1
-
-
-
-
4
-
4
2
2
-
9
-
-
1
-
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
2
2
-
-
-
1
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
1
1
-
-
-
726601
Arnfors
Expression, purification, crys ...
Methanocaldococcus jannaschii
Acta Crystallogr. Sect. F
61
591-594
2005
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-