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Expression, purification, crystallization and preliminary X-ray analysis of a nucleoside kinase from the hyperthermophile Methanocaldococcus jannaschii

Arnfors, L.; Hansen, T.; Meining, W.; Schönheit, P.; Ladenstein, R.; Acta Crystallogr. Sect. F 61, 591-594 (2005)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
crystallized as the apoenzyme as well as in complex with an ATP analogue and Mg2+. The latter crystal form is also soaked with D-fructose 6-phosphate. Synchrotron-radiation data are collected to 1.70 A for the apoenzyme crystals and 1.93 A for the complex crystals. All crystals exhibit orthorhombic symmetry. The apoenzyme crystals contain one monomer per asymmetric unit whereas the complex crystals contain a dimer
Methanocaldococcus jannaschii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Methanocaldococcus jannaschii
Q57849
-
-
Purification (Commentary)
Commentary
Organism
-
Methanocaldococcus jannaschii
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystallized as the apoenzyme as well as in complex with an ATP analogue and Mg2+. The latter crystal form is also soaked with D-fructose 6-phosphate. Synchrotron-radiation data are collected to 1.70 A for the apoenzyme crystals and 1.93 A for the complex crystals. All crystals exhibit orthorhombic symmetry. The apoenzyme crystals contain one monomer per asymmetric unit whereas the complex crystals contain a dimer
Methanocaldococcus jannaschii
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methanocaldococcus jannaschii
Other publictions for EC 2.7.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728780
Elkin
A broad specificity nucleoside ...
Thermoplasma acidophilum
Proteins
81
568-582
2013
-
-
1
1
-
-
-
6
1
3
2
-
-
4
-
-
1
-
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
6
1
3
2
-
-
-
-
1
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
6
6
697882
Ota
A novel nucleoside kinase from ...
Burkholderia thailandensis, Burkholderia thailandensis DSM 13276
FEBS J.
275
5865-5872
2008
-
-
1
-
-
-
2
8
-
3
2
-
-
7
-
-
1
-
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
8
-
3
2
-
-
-
-
1
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
8
8
726575
Arnfors
Structure of Methanocaldococcu ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Acta Crystallogr. Sect. D
62
1085-1097
2006
-
-
-
1
-
-
-
-
-
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727430
Hansen
The phosphofructokinase-B (MJ0 ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Extremophiles
11
105-114
2006
-
-
1
-
-
-
-
4
-
4
2
2
-
9
-
-
1
-
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
2
2
-
-
-
1
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
1
1
-
-
-
726601
Arnfors
Expression, purification, crys ...
Methanocaldococcus jannaschii
Acta Crystallogr. Sect. F
61
591-594
2005
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-
-
1
-
-
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-
-
-
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-
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2
-
-
1
-
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-
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1
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1
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