BRENDA - Enzyme Database
show all sequences of 2.7.1.B20

A novel nucleoside kinase from Burkholderia thailandensis: a member of the phosphofructokinase B-type family of enzymes

Ota, H.; Sakasegawa, S.; Yasuda, Y.; Imamura, S.; Tamura, T.; FEBS J. 275, 5865-5872 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
functionally overexpressed in Rhodococcus erythropolis
Burkholderia thailandensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
adenosine
concentrations above 0.1 mM lead to reduction in enzyme activity
Burkholderia thailandensis
Inosine
concentrations above 0.1 mM lead to reduction in enzyme activity
Burkholderia thailandensis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
adenosine
pH 7.0, 37C
Burkholderia thailandensis
0.15
-
Inosine
pH 7.0, 37C
Burkholderia thailandensis
0.46
-
guanosine
pH 7.0, 37C
Burkholderia thailandensis
0.6
3
ATP
pH 7.0, 37C
Burkholderia thailandensis
0.79
-
ribavirin
pH 7.0, 37C
Burkholderia thailandensis
0.87
-
mizoribine
pH 7.0, 37C
Burkholderia thailandensis
1.1
-
2'-deoxyadenosine
pH 7.0, 37C
Burkholderia thailandensis
1.7
-
uridine
pH 7.0, 37C
Burkholderia thailandensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+
Burkholderia thailandensis
Mg2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+. Maximum activity is attained when the ATP/Mg2+ concentration ratio is 0.5
Burkholderia thailandensis
Ni2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+
Burkholderia thailandensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 34000, SDS-PAGE
Burkholderia thailandensis
64000
-
gel filtration
Burkholderia thailandensis
Organism
Organism
UniProt
Commentary
Textmining
Burkholderia thailandensis
Q2SZE4
-
-
Burkholderia thailandensis DSM 13276
Q2SZE4
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Burkholderia thailandensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 2'-deoxyadenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + adenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + adenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + guanosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + guanosine 5'-phosphate
-
-
-
?
ATP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + inosine 5'-phosphate
-
-
-
?
ATP + mizoribine
i.e. 5-hydroxy-1-beta-D-ribofuranosyl-1H-imidazole-4-carboxamide. Phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + mizoribine 5'-phosphate
-
-
-
?
ATP + ribavirin
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + ribavirin 5'-phosphate
-
-
-
?
ATP + ribavirin
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + ribavirin 5'-phosphate
-
-
-
?
ATP + uridine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + uridine 5'-phosphate
-
-
-
?
ATP + uridine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + uridine 5'-phosphate
-
-
-
?
CTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
CDP + inosine 5'-phosphate
-
-
-
?
GTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
GDP + inosine 5'-phosphate
-
-
-
?
ITP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
IDP + inosine 5'-phosphate
-
-
-
?
additional information
in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine
697882
Burkholderia thailandensis
?
-
-
-
-
additional information
in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine
697882
Burkholderia thailandensis DSM 13276
?
-
-
-
-
TTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
TDP + inosine 5'-phosphate
-
-
-
?
UTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
UDP + inosine 5'-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 34000, SDS-PAGE
Burkholderia thailandensis
Synonyms
Synonyms
Commentary
Organism
BTH_I1158
locus name
Burkholderia thailandensis
nucleoside kinase
-
Burkholderia thailandensis
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
35
-
pH 7.0, 20 min, stable
Burkholderia thailandensis
60
-
pH 7.0, 20 min, 60% loss of activity
Burkholderia thailandensis
70
-
pH 7.0, 20 min, complete inactivation
Burkholderia thailandensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
37C, 3 h, stable
Burkholderia thailandensis
Cloned(Commentary) (protein specific)
Commentary
Organism
functionally overexpressed in Rhodococcus erythropolis
Burkholderia thailandensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
adenosine
concentrations above 0.1 mM lead to reduction in enzyme activity
Burkholderia thailandensis
Inosine
concentrations above 0.1 mM lead to reduction in enzyme activity
Burkholderia thailandensis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
adenosine
pH 7.0, 37C
Burkholderia thailandensis
0.15
-
Inosine
pH 7.0, 37C
Burkholderia thailandensis
0.46
-
guanosine
pH 7.0, 37C
Burkholderia thailandensis
0.6
3
ATP
pH 7.0, 37C
Burkholderia thailandensis
0.79
-
ribavirin
pH 7.0, 37C
Burkholderia thailandensis
0.87
-
mizoribine
pH 7.0, 37C
Burkholderia thailandensis
1.1
-
2'-deoxyadenosine
pH 7.0, 37C
Burkholderia thailandensis
1.7
-
uridine
pH 7.0, 37C
Burkholderia thailandensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+
Burkholderia thailandensis
Mg2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+. Maximum activity is attained when the ATP/Mg2+ concentration ratio is 0.5
Burkholderia thailandensis
Ni2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+
Burkholderia thailandensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
2 * 34000, SDS-PAGE
Burkholderia thailandensis
64000
-
gel filtration
Burkholderia thailandensis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Burkholderia thailandensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 2'-deoxyadenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + adenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + adenosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + adenosine 5'-phosphate
-
-
-
?
ATP + guanosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + guanosine 5'-phosphate
-
-
-
?
ATP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + inosine 5'-phosphate
-
-
-
?
ATP + mizoribine
i.e. 5-hydroxy-1-beta-D-ribofuranosyl-1H-imidazole-4-carboxamide. Phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + mizoribine 5'-phosphate
-
-
-
?
ATP + ribavirin
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + ribavirin 5'-phosphate
-
-
-
?
ATP + ribavirin
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + ribavirin 5'-phosphate
-
-
-
?
ATP + uridine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
ADP + uridine 5'-phosphate
-
-
-
?
ATP + uridine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis DSM 13276
ADP + uridine 5'-phosphate
-
-
-
?
CTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
CDP + inosine 5'-phosphate
-
-
-
?
GTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
GDP + inosine 5'-phosphate
-
-
-
?
ITP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
IDP + inosine 5'-phosphate
-
-
-
?
additional information
in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine
697882
Burkholderia thailandensis
?
-
-
-
-
additional information
in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine
697882
Burkholderia thailandensis DSM 13276
?
-
-
-
-
TTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
TDP + inosine 5'-phosphate
-
-
-
?
UTP + inosine
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
697882
Burkholderia thailandensis
UDP + inosine 5'-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 34000, SDS-PAGE
Burkholderia thailandensis
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
35
-
pH 7.0, 20 min, stable
Burkholderia thailandensis
60
-
pH 7.0, 20 min, 60% loss of activity
Burkholderia thailandensis
70
-
pH 7.0, 20 min, complete inactivation
Burkholderia thailandensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
10
37C, 3 h, stable
Burkholderia thailandensis
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.04
-
mizoribine
pH 7.0, 37C
Burkholderia thailandensis
0.08
-
ribavirin
pH 7.0, 37C
Burkholderia thailandensis
0.24
-
guanosine
pH 7.0, 37C
Burkholderia thailandensis
0.28
-
uridine
pH 7.0, 37C
Burkholderia thailandensis
0.84
-
2'-deoxyadenosine
pH 7.0, 37C
Burkholderia thailandensis
6.05
-
adenosine
pH 7.0, 37C
Burkholderia thailandensis
16
-
ATP
pH 7.0, 37C
Burkholderia thailandensis
80
-
Inosine
pH 7.0, 37C
Burkholderia thailandensis
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.04
-
mizoribine
pH 7.0, 37C
Burkholderia thailandensis
0.08
-
ribavirin
pH 7.0, 37C
Burkholderia thailandensis
0.24
-
guanosine
pH 7.0, 37C
Burkholderia thailandensis
0.28
-
uridine
pH 7.0, 37C
Burkholderia thailandensis
0.84
-
2'-deoxyadenosine
pH 7.0, 37C
Burkholderia thailandensis
6.05
-
adenosine
pH 7.0, 37C
Burkholderia thailandensis
16
-
ATP
pH 7.0, 37C
Burkholderia thailandensis
80
-
Inosine
pH 7.0, 37C
Burkholderia thailandensis
Other publictions for EC 2.7.1.B20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728780
Elkin
A broad specificity nucleoside ...
Thermoplasma acidophilum
Proteins
81
568-582
2013
-
-
1
1
-
-
-
6
1
3
2
-
-
4
-
-
1
-
-
-
-
-
7
1
1
1
-
-
6
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
6
1
3
2
-
-
-
-
1
-
-
-
-
7
1
1
-
-
6
2
1
-
-
-
-
-
-
6
6
697882
Ota
A novel nucleoside kinase from ...
Burkholderia thailandensis, Burkholderia thailandensis DSM 13276
FEBS J.
275
5865-5872
2008
-
-
1
-
-
-
2
8
-
3
2
-
-
7
-
-
1
-
-
-
-
-
18
1
2
-
-
3
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
2
-
8
-
3
2
-
-
-
-
1
-
-
-
-
18
1
-
-
3
-
-
-
1
-
-
-
-
-
8
8
726575
Arnfors
Structure of Methanocaldococcu ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Acta Crystallogr. Sect. D
62
1085-1097
2006
-
-
-
1
-
-
-
-
-
1
-
-
-
8
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727430
Hansen
The phosphofructokinase-B (MJ0 ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661
Extremophiles
11
105-114
2006
-
-
1
-
-
-
-
4
-
4
2
2
-
9
-
-
1
-
-
-
-
-
16
1
3
1
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
2
2
-
-
-
1
-
-
-
-
16
1
1
-
1
-
1
1
-
-
-
1
1
-
-
-
726601
Arnfors
Expression, purification, crys ...
Methanocaldococcus jannaschii
Acta Crystallogr. Sect. F
61
591-594
2005
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-