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Literature summary for 2.7.1.B19 extracted from
- Identification and characterization of geranylgeraniol kinase and geranylgeranyl phosphate kinase from the archaebacterium Sulfolobus acidocaldarius (1996), J. Biochem., 119, 541-547.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Sulfolobus acidocaldarius |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000027 | - |
geranylgeraniol | pH 7.0, 55°C, assay with membrane fraction | Sulfolobus acidocaldarius | |
| 0.65 | - |
ATP | pH 7.0, 55°C, assay with membrane fraction | Sulfolobus acidocaldarius | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Sulfolobus acidocaldarius | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
| Mg2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
| Mn2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
| Ni2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
| Zn2+ | 5 mM, activates. No activity in absence of cations | Sulfolobus acidocaldarius | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + geranylgeraniol | Sulfolobus acidocaldarius | all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation | ADP + geranylgeranyl phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (2E,6E)-farnesol | 7% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | ADP + (2E,6E)-farnesyl phosphate | - |
? | |
| ATP + all-trans-hexaprenol | 38% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | ADP + all-trans-hexaprenyl phosphate | - |
? | |
| ATP + all-trans-octaprenol | 24% of the activity compared to all-trans-geranylgeraniol. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | ADP + all-trans-octaprenyl phosphate | - |
? | |
| ATP + geranylgeraniol | all-trans-geranylgeraniol seems to be converted to its monophosphate in the membrane, and this product moves to the cytosol to undergo a second phosphorylation | Sulfolobus acidocaldarius | ADP + geranylgeranyl phosphate | - |
? | |
| ATP + geranylgeraniol | all-trans-geranylgeraniol and ATP are the best substrates, activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes. No formation of geranylgeranyl diphosphate is observed | Sulfolobus acidocaldarius | ADP + geranylgeranyl phosphate | - |
? | |
| CTP + geranylgeraniol | 63% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | CDP + geranylgeranyl phosphate | - |
? | |
| GTP + geranylgeraniol | 87% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | GDP + geranylgeranyl phosphate | - |
? | |
| additional information | neither geraniol nor (22E,6E,10Z,14Z,18Z,22Z,26Z,30Z,34Z,38Z)-undecaprenol are active as substrates | Sulfolobus acidocaldarius | ? | - |
? | |
| UTP + geranylgeraniol | 76% of the activity compared to ATP. Activity is tested with membrane fraction washed with buffer to avoid contamination with soluble enzymes | Sulfolobus acidocaldarius | UDP + geranylgeranyl phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GGOH kinase | - |
Sulfolobus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at | Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | activity at 70% is about 40% of the activity at 90°C, no activity below 40°C | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
1 h, about 15% loss of activity | Sulfolobus acidocaldarius |
| 95 | - |
1 h, more than 90% loss of activity | Sulfolobus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Sulfolobus acidocaldarius |
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