Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes | Bos taurus | |
Phospholipids | e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate | Bos taurus |
General Stability | Organism |
---|---|
DTT, EDTA or ATP stabilizes during purification | Bos taurus |
protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | DTT prevents inhibition | Bos taurus | |
NEM | DTT prevents inhibition | Bos taurus | |
p-chloromercuriphenylsulfonate | DTT prevents inhibition | Bos taurus | |
phosphatidylcholine | inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate | Bos taurus | |
phosphatidylethanolamine | inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate | Bos taurus | |
sphingomyelin | inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 58% as effective as Mg2+ in activation | Bos taurus | |
Co2+ | 5 mM, 60% as effective as Mg2+ in activation | Bos taurus | |
Cu2+ | 5 mM, 51% as effective as Mg2+ in activation | Bos taurus | |
Fe2+ | 5 mM, 60% as effective as Mg2+ in activation | Bos taurus | |
Mg2+ | required | Bos taurus | |
Mg2+ | optimal concentration is 10 mM | Bos taurus | |
Mn2+ | 5 mM, as effective as Mg2+ in activation | Bos taurus | |
Ni2+ | 5 mM, 60% as effective as Mg2+ in activation | Bos taurus | |
Zn2+ | 5 mM, 48% as effective as Mg2+ in activation | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-arachidonoylglycerol | - |
Bos taurus | ADP + 1-arachidonoylglycerol 3-phosphate | - |
? | |
ATP + 1-arachidoylglycerol | - |
Bos taurus | ADP + 1-arachidoylglycerol 3-phosphate | - |
? | |
ATP + 1-caproylglycerol | - |
Bos taurus | ADP + 1-caproylglycerol 3-phosphate | - |
? | |
ATP + 1-capryloylglycerol | - |
Bos taurus | ADP + 1-capryloylglycerol 3-phosphate | - |
? | |
ATP + 1-lauroylglycerol | - |
Bos taurus | ADP + 1-lauroylglycerol 3-phosphate | - |
? | |
ATP + 1-linolenoylglycerol | - |
Bos taurus | ADP + 1-linolenoylglycerol 3-phosphate | - |
? | |
ATP + 1-linoleoylglycerol | - |
Bos taurus | ADP + 1-linoleoylglycerol 3-phosphate | - |
? | |
ATP + 1-monoolein | i.e. 1-monooleolylglyerol | Bos taurus | ADP + 1-oleoylglycerol 3-phosphate | - |
? | |
ATP + 1-myristoylglycerol | - |
Bos taurus | ADP + 1-myristoylglycerol 3-phosphate | - |
? | |
ATP + 1-palmitoleoylglycerol | - |
Bos taurus | ADP + 1-palmitoleoylglycerol 3-phosphate | - |
? | |
ATP + 1-palmitoylglycerol | - |
Bos taurus | ADP + 1-palmitoylglycerol 3-phosphate | - |
? | |
ATP + 1-stearoylglycerol | - |
Bos taurus | ADP + 1-stearoylglycerol 3-phosphate | - |
? | |
ATP + 2-arachidonoylglycerol | - |
Bos taurus | ADP + 2-arachidonoylglycerol 3-phosphate | - |
? | |
ATP + 2-arachidoylglycerol | - |
Bos taurus | ADP + 2-arachidoylglycerol 3-phosphate | - |
? | |
ATP + 2-caproylglycerol | - |
Bos taurus | ADP + 2-caproylglycerol 3-phosphate | - |
? | |
ATP + 2-lauroylglycerol | - |
Bos taurus | ADP + 2-lauroylglycerol 3-phosphate | - |
? | |
ATP + 2-linolenoylglycerol | - |
Bos taurus | ADP + 2-linolenoylglycerol 3-phosphate | - |
? | |
ATP + 2-linoleoylglycerol | - |
Bos taurus | ADP + 2-linoleoylglycerol 3-phosphate | - |
? | |
ATP + 2-monoolein | i.e. 2-monooleolylglyerol | Bos taurus | ADP + 2-oleoyoglycerol 3-phosphate | - |
? | |
ATP + 2-palmitoleoylglycerol | - |
Bos taurus | ADP + 2-palmitoleoylglycerol 3-phosphate | - |
? | |
ATP + 2-palmitoylglycerol | - |
Bos taurus | ADP + 2-palmitoylglycerol 3-phosphate | - |
? | |
ATP + 2-stearoylglycerol | - |
Bos taurus | ADP + 2-stearoylglycerol 3-phosphate | - |
? | |
additional information | 1-monostearin amongst the 1-acylglycerols and 2-monoarachidonin amongst the 2-acylglycerols give the highest activity | Bos taurus | ? | - |
? | |
additional information | preference for substrates with unsaturated fatty acids except for 1- and 2-monostearins | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000 | Bos taurus |