Biosynthesis of 1-alkyl-sn-glycero-3-phosphate via adenosine triphosphate:1-alkyl-sn-glycerol phosphotransferase

Rock, C.O.; Snyder, F.; J. Biol. Chem. 249, 5382-5387 (1974)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

1.6

-

ATP

-

Oryctolagus cuniculus

Metals/Ions

Commentary

Organism

Structure

Mg2+

absolute requirement for a divalent metal ion, Mg2+ is most effective

Oryctolagus cuniculus

Mn2+

about 50% as effective as Mg2+ in activation

Oryctolagus cuniculus

additional information

little activity with Zn2+, Ba2+, Cu2+, Ca2+, Hg2+, Sn2+, Sr2+, Ni2+, Fe2+, NaCl and KCl

Oryctolagus cuniculus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + 1-alkyl-sn-glycerol

Oryctolagus cuniculus

involved in the biosynthesis of complex ether-linked glycerolipids

ADP + 1-alkyl-sn-glycero-3-phosphate

-

Oryctolagus cuniculus

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

ATP + 1-alkyl-sn-glycerol

stereoselective transfer of the terminal phosphate of ATP to position 3 of the substrate, ATP is an absolute requirement for enzymatic activity

642123

Oryctolagus cuniculus

ADP + 1-alkyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

ATP + 1-alkyl-sn-glycerol

involved in the biosynthesis of complex ether-linked glycerolipids

642123

Oryctolagus cuniculus

ADP + 1-alkyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

ATP + 1-O-hexadecyl-sn-glycerol

-

642123

Oryctolagus cuniculus

ADP + 1-O-hexadecyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

ATP + 1-octadecyl-sn-glycerol

-

642123

Oryctolagus cuniculus

ADP + 1-octadecyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

additional information

not: alkylethylene glycols, only trace activity with 1-thio-hexadecyl-2,3-propanediol

642123

Oryctolagus cuniculus

?

-

-

-

?

additional information

not: S-alkyl-alkylglycerols, 2-hexadecylglycerol, 3-hexadecyl-sn-glycerol, 1-hexadecylethylene glycol

642123

Oryctolagus cuniculus

?

-

-

-

?

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

1.6

-

ATP

-

Oryctolagus cuniculus

Metals/Ions

Commentary

Organism

Structure

Mg2+

absolute requirement for a divalent metal ion, Mg2+ is most effective

Oryctolagus cuniculus

Mn2+

about 50% as effective as Mg2+ in activation

Oryctolagus cuniculus

additional information

little activity with Zn2+, Ba2+, Cu2+, Ca2+, Hg2+, Sn2+, Sr2+, Ni2+, Fe2+, NaCl and KCl

Oryctolagus cuniculus

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

ATP + 1-alkyl-sn-glycerol

Oryctolagus cuniculus

involved in the biosynthesis of complex ether-linked glycerolipids

ADP + 1-alkyl-sn-glycero-3-phosphate

-

Oryctolagus cuniculus

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

ATP + 1-alkyl-sn-glycerol

stereoselective transfer of the terminal phosphate of ATP to position 3 of the substrate, ATP is an absolute requirement for enzymatic activity

642123

Oryctolagus cuniculus

ADP + 1-alkyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

ATP + 1-alkyl-sn-glycerol

involved in the biosynthesis of complex ether-linked glycerolipids

642123

Oryctolagus cuniculus

ADP + 1-alkyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

ATP + 1-O-hexadecyl-sn-glycerol

-

642123

Oryctolagus cuniculus

ADP + 1-O-hexadecyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

ATP + 1-octadecyl-sn-glycerol

-

642123

Oryctolagus cuniculus

ADP + 1-octadecyl-sn-glycero-3-phosphate

-

642123

Oryctolagus cuniculus

?

additional information

not: alkylethylene glycols, only trace activity with 1-thio-hexadecyl-2,3-propanediol

642123

Oryctolagus cuniculus

?

-

-

-

?

additional information

not: S-alkyl-alkylglycerols, 2-hexadecylglycerol, 3-hexadecyl-sn-glycerol, 1-hexadecylethylene glycol

642123

Oryctolagus cuniculus

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

642124

Snyder

Alkylglycerol phosphotransfera ...

Oryctolagus cuniculus

Methods Enzymol.

209

211-215

1992

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

-

1

-

-

1

-

1

5

-

-

1

-

-

-

1

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

1

-

1

5

-

1

-

-

-

1

1

-

-

-

-

-

-

-

-

642123

Rock

Biosynthesis of 1-alkyl-sn-gly ...

Oryctolagus cuniculus

J. Biol. Chem.

249

5382-5387

1974

-

-

-

-

-

-

1

1

1

3

-

1

-

1

-

-

1

-

-

1

-

1

6

-

-

1

-

-

-

2

1

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

1

1

3

-

1

-

-

-

1

-

1

-

1

6

-

1

-

-

-

2

1

-

-

-

-

-

-

-

-