BRENDA - Enzyme Database
show all sequences of 2.7.1.93

Biosynthesis of 1-alkyl-sn-glycero-3-phosphate via adenosine triphosphate:1-alkyl-sn-glycerol phosphotransferase

Rock, C.O.; Snyder, F.; J. Biol. Chem. 249, 5382-5387 (1974) View publication on PubMed

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by rac-1-hexadecanoylglycerol, rac-1,2-dihexadecanoylglycerol, rac-1-hexadecyl-2-hexadecanoylglycerol or 2-hexadecylglycerol
Oryctolagus cuniculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
ATP
-
Oryctolagus cuniculus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement for a divalent metal ion, Mg2+ is most effective
Oryctolagus cuniculus
Mn2+
about 50% as effective as Mg2+ in activation
Oryctolagus cuniculus
additional information
little activity with Zn2+, Ba2+, Cu2+, Ca2+, Hg2+, Sn2+, Sr2+, Ni2+, Fe2+, NaCl and KCl
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 1-alkyl-sn-glycerol
Oryctolagus cuniculus
involved in the biosynthesis of complex ether-linked glycerolipids
ADP + 1-alkyl-sn-glycero-3-phosphate
-
Oryctolagus cuniculus
?
Organism
Organism
UniProt
Commentary
Textmining
Oryctolagus cuniculus
-
adult male New Zealand white rabbit
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Oryctolagus cuniculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
harderian gland
pink portions of
Oryctolagus cuniculus
-
Storage Stability
Storage Stability
Organism
-20°C, whole microsomal preparation, 0.25 M sucrose, 5 mM dithiothreitol, at least 2 months, stable
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + 1-alkyl-sn-glycerol
stereoselective transfer of the terminal phosphate of ATP to position 3 of the substrate, ATP is an absolute requirement for enzymatic activity
642123
Oryctolagus cuniculus
ADP + 1-alkyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
ATP + 1-alkyl-sn-glycerol
involved in the biosynthesis of complex ether-linked glycerolipids
642123
Oryctolagus cuniculus
ADP + 1-alkyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
ATP + 1-O-hexadecyl-sn-glycerol
-
642123
Oryctolagus cuniculus
ADP + 1-O-hexadecyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
ATP + 1-octadecyl-sn-glycerol
-
642123
Oryctolagus cuniculus
ADP + 1-octadecyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
additional information
not: alkylethylene glycols, only trace activity with 1-thio-hexadecyl-2,3-propanediol
642123
Oryctolagus cuniculus
?
-
-
-
?
additional information
not: S-alkyl-alkylglycerols, 2-hexadecylglycerol, 3-hexadecyl-sn-glycerol, 1-hexadecylethylene glycol
642123
Oryctolagus cuniculus
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
0.1 M Tris-maleate buffer
Oryctolagus cuniculus
7.1
-
no sharp pH optimum
Oryctolagus cuniculus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.1
8.1
about 66% of maximal activity at pH 6.1 and 8.1
Oryctolagus cuniculus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
not inhibited by rac-1-hexadecanoylglycerol, rac-1,2-dihexadecanoylglycerol, rac-1-hexadecyl-2-hexadecanoylglycerol or 2-hexadecylglycerol
Oryctolagus cuniculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.6
-
ATP
-
Oryctolagus cuniculus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Oryctolagus cuniculus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
absolute requirement for a divalent metal ion, Mg2+ is most effective
Oryctolagus cuniculus
Mn2+
about 50% as effective as Mg2+ in activation
Oryctolagus cuniculus
additional information
little activity with Zn2+, Ba2+, Cu2+, Ca2+, Hg2+, Sn2+, Sr2+, Ni2+, Fe2+, NaCl and KCl
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ATP + 1-alkyl-sn-glycerol
Oryctolagus cuniculus
involved in the biosynthesis of complex ether-linked glycerolipids
ADP + 1-alkyl-sn-glycero-3-phosphate
-
Oryctolagus cuniculus
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Oryctolagus cuniculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
harderian gland
pink portions of
Oryctolagus cuniculus
-
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, whole microsomal preparation, 0.25 M sucrose, 5 mM dithiothreitol, at least 2 months, stable
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + 1-alkyl-sn-glycerol
stereoselective transfer of the terminal phosphate of ATP to position 3 of the substrate, ATP is an absolute requirement for enzymatic activity
642123
Oryctolagus cuniculus
ADP + 1-alkyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
ATP + 1-alkyl-sn-glycerol
involved in the biosynthesis of complex ether-linked glycerolipids
642123
Oryctolagus cuniculus
ADP + 1-alkyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
ATP + 1-O-hexadecyl-sn-glycerol
-
642123
Oryctolagus cuniculus
ADP + 1-O-hexadecyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
ATP + 1-octadecyl-sn-glycerol
-
642123
Oryctolagus cuniculus
ADP + 1-octadecyl-sn-glycero-3-phosphate
-
642123
Oryctolagus cuniculus
?
additional information
not: alkylethylene glycols, only trace activity with 1-thio-hexadecyl-2,3-propanediol
642123
Oryctolagus cuniculus
?
-
-
-
?
additional information
not: S-alkyl-alkylglycerols, 2-hexadecylglycerol, 3-hexadecyl-sn-glycerol, 1-hexadecylethylene glycol
642123
Oryctolagus cuniculus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Oryctolagus cuniculus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.1
-
0.1 M Tris-maleate buffer
Oryctolagus cuniculus
7.1
-
no sharp pH optimum
Oryctolagus cuniculus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.1
8.1
about 66% of maximal activity at pH 6.1 and 8.1
Oryctolagus cuniculus
Other publictions for EC 2.7.1.93
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
642124
Snyder
Alkylglycerol phosphotransfera ...
Oryctolagus cuniculus
Methods Enzymol.
209
211-215
1992
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
1
5
-
-
1
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
1
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
642123
Rock
Biosynthesis of 1-alkyl-sn-gly ...
Oryctolagus cuniculus
J. Biol. Chem.
249
5382-5387
1974
-
-
-
-
-
-
1
1
1
3
-
1
-
1
-
-
1
-
-
1
-
1
6
-
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
3
-
1
-
-
-
1
-
1
-
1
6
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-