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Literature summary for 2.7.1.92 extracted from

  • Anderson, W.A.; Magasanik, B.
    The pathway of myo-inositol degradation in Aerobacter aerogenes. Conversion of 2-deoxy-5-keto-D-gluconic acid to glycolytic intermediates (1971), J. Biol. Chem., 246, 5662-5675.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme is induced by growth on myo-inositol Klebsiella aerogenes

General Stability

General Stability Organism
purified enzyme is unstable to repeated freezing and thawing in 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.097
-
2-deoxy-5-keto-D-gluconic acid
-
Klebsiella aerogenes
0.22
-
ATP
-
Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 is included in assay mixture on the assumption that it is required Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
225000
-
sucrose density gradient centrifugation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2-deoxy-5-keto-D-gluconic acid Klebsiella aerogenes involved in myo-inositol degradation ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
ATP + 2-deoxy-5-keto-D-gluconic acid Klebsiella aerogenes constitutive involved in myo-inositol degradation ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
C1, constitutive strain
-
Klebsiella aerogenes constitutive
-
C1, constitutive strain
-

Purification (Commentary)

Purification (Comment) Organism
partial, 20fold, not seperated from 2-deoxy-5-keto-D-gluconic acid 6-phosphate aldolase Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.6
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
0°C, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 1 month or more, stable Klebsiella aerogenes
4°C, enzyme of DEAE-Sephadex fractions, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 0.2 M KCl, 5 months, stable Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-deoxy-5-keto-D-gluconic acid
-
Klebsiella aerogenes ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
ATP + 2-deoxy-5-keto-D-gluconic acid involved in myo-inositol degradation Klebsiella aerogenes ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
ATP + 2-deoxy-5-keto-D-gluconic acid
-
Klebsiella aerogenes constitutive ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
ATP + 2-deoxy-5-keto-D-gluconic acid involved in myo-inositol degradation Klebsiella aerogenes constitutive ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8.5 broad, Tris-chloride buffer Klebsiella aerogenes
8
-
assay at Klebsiella aerogenes