Literature summary for 2.7.1.91 extracted from

Nishino, S.; Yamashita, H.; Tamori, M.; Mashimo, M.; Yamagata, K.; Nakamura, H.; Murayama, T.
Translocation and activation of sphingosine kinase 1 by ceramide-1-phosphate (2019), J. Cell. Biochem., 120, 5396-5408 .

Search for more literature for 2.7.1.91

Activating Compound

Activating Compound	Comment	Organism	Structure
ceramide 1-phosphate	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells and HEK-293T cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R56A/R57A/H59A/R61A	the mutations of isoform SphK1 reduce the phorbol 12-myristate 13-acetate- and ceramide 1-phosphate-induced translocation of isoform SphK1 to the plasma membrane, however, the capacity of ceramide 1-phosphate to bind with and activate isoform SphK1 is not affected by the mutations	Homo sapiens
S225A	the mutant is not able to translocate to the plasma membrane	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plasma membrane	-	Homo sapiens	5886	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + sphingosine	Homo sapiens	-	ADP + sphingosine 1-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9NYA1	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	the translocation and activity of isoform SphK1 are regulated by its phosphorylation of Ser225	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose bead chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + sphingosine	-	Homo sapiens	ADP + sphingosine 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
sphingosine kinase 1	-	Homo sapiens
SPHK1	isoform	Homo sapiens

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Release 2023.1 (January 2023)