Cloned (Comment) | Organism |
---|---|
quantitative real-time reverse transcriptase-PCR expression analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockdown in HeLa cells using siRNA cell treatment. Enzyme knockdown significantly enhances RANTES induction in response to TNF with no statistically significant effect on unstimulated levels. RANTES induction is highly NF-kappaB-dependent as shown using NF-kappaB inhibitor BAY 11-7082 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sphingosine | Homo sapiens | - |
ADP + sphingosine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NYA1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + sphingosine | - |
Homo sapiens | ADP + sphingosine 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SK1 | - |
Homo sapiens |
sphingosine kinase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of sphingosine kinase 1 potentiates induction of the chemokine RANTES (regulated on activation, normal T cell expressed and secreted, also known as CCL5) in HeLa cells stimulated with TNF-alpha despite RANTES induction being highly dependent on the NF-kappaB pathway. Analysis of the mechanism by which SK1 regulates RANTES induction, overview. Loss of SK1 strongly upregulates RANTES independently of effects on NF-kappaB activation, while loss of SK2 does not affect NF-kappaB pathway activation | Homo sapiens |
physiological function | sphingosine kinase 1 produces the pro-survival sphingolipid sphingosine 1-phosphate and is implicated in inflammation, proliferation, and angiogenesis. The enzyme is required for activation of p38 MAPK, but is not required for transcriptional NF-kappaB activity following TNF treatment. But nuclear translocation of NF-kappaB subunits does not require enzyme SK1 | Homo sapiens |