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Literature summary for 2.7.1.91 extracted from
- Arabidopsis sphingosine kinase and the effects of phytosphingosine-1-phosphate on stomatal aperture (2005), Plant Physiol., 137, 724-737.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | the enzyme activity is higher with substrate solubilized by bovine serum albumin compared to Triton X-100 | Arabidopsis thaliana | |
| additional information | the enzyme is stimulated by the phytohormone abscisic acid in guard cells and mesophyll cell protoplasts | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic analysis | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-threo-dihydrosphingosine | slight inhibition | Arabidopsis thaliana |  |
| N,N-dimethylsphingosine | strong inhibition | Arabidopsis thaliana | |
| Triton X-100 | the enzyme activity is higher with substrate solubilized by bovine serum albumin compared to Triton X-100 | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | the cytosolic enzyme is 20fold less active than the membrane-associated enzyme | Arabidopsis thaliana | 5829 | - |
| membrane | 75% of total activity in leaves, associated with, the cytosolic enzyme is 20fold less active than the membrane-associated enzyme | Arabidopsis thaliana | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phytosphingosine | Arabidopsis thaliana | phytosphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins | ADP + phytosphingosine 1-phosphate | - |
ir | |
| ATP + sphingosine | Arabidopsis thaliana | sphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins | ADP + sphingosine 1-phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
several isozymes | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| guard cell | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| mesophyll | - |
Arabidopsis thaliana | - |
| stoma | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 4,8-sphingadienine | high activity | Arabidopsis thaliana | ADP + 4,8-sphingadienine 1-phosphate | - |
ir | |
| ATP + DELTA4-unsaturated long chain sphingosine | high activity | Arabidopsis thaliana | ADP + DELTA4-unsaturated long chain sphingosine 1-phosphate | - |
ir | |
| ATP + phytosphingosine | phytosphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins | Arabidopsis thaliana | ADP + phytosphingosine 1-phosphate | - |
ir | |
| ATP + phytosphingosine | i.e. 4-hydroxysphinganine, moderate or low activity | Arabidopsis thaliana | ADP + phytosphingosine 1-phosphate | - |
ir | |
| ATP + sphinganine | i.e. dihydrosphingosine, moderate or low activity | Arabidopsis thaliana | ADP + sphinganine 1-phosphate | - |
ir | |
| ATP + sphingosine | - |
Arabidopsis thaliana | ADP + sphingosine 1-phosphate | - |
ir | |
| ATP + sphingosine | sphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins | Arabidopsis thaliana | ADP + sphingosine 1-phosphate | - |
ir | |
| additional information | substrate specificity of cytosolic and membrane enzymes, 4-hydroxy-8-sphingenine is a poor substrate | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sphinganine kinase | - |
Arabidopsis thaliana |
| SPHK | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
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